| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP2K3 |
| Uniprot No | P46734 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-347aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSKPPAPNPT PPRNLDSRTF ITIGDRNFEV EADDLVTISE LGRGAYGVVE KVRHAQSGTI MAVKRIRATV NSQEQKRLLM DLDINMRTVD CFYTVTFYGA LFREGDVWIC MELMDTSLDK FYRKVLDKNM TIPEDILGEI AVSIVRALEH LHSKLSVIHR DVKPSNVLIN KEGHVKMCDF GISGYLVDSV AKTMDAGCKP YMAPERINPE LNQKGYNVKS DVWSLGITMI EMAILRFPYE SWGTPFQQLK QVVEEPSPQL PADRFSPEFV DFTAQCLRKN PAERMSYLEL MEHPFFTLHK TKKTDIAAFV KEILGEDS |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAP2K3 Recombinant Protein**
MAP2K3 (Mitogen-activated protein kinase kinase 3), also known as MEK3. is a dual-specificity serine/threonine and tyrosine kinase that plays a critical role in cellular signaling pathways. It functions as a key component of the p38 mitogen-activated protein kinase (MAPK) cascade, which is involved in regulating responses to stress signals, cytokines, and growth factors. MAP2K3 directly phosphorylates and activates p38 MAPK, which subsequently modulates downstream targets influencing processes like inflammation, apoptosis, cell differentiation, and immune responses. Dysregulation of this pathway has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
Recombinant MAP2K3 protein is engineered using expression systems such as *E. coli* or mammalian cells to produce a purified, biologically active form of the enzyme. This recombinant tool retains the kinase activity required for *in vitro* studies, enabling researchers to investigate MAPK signaling mechanisms, screen for inhibitors or activators, and analyze protein-protein interactions. Its structure typically includes conserved catalytic domains critical for substrate recognition and ATP binding, ensuring functional relevance to native MAP2K3.
The availability of recombinant MAP2K3 has advanced drug discovery, particularly in targeting inflammatory and oncogenic pathways. It also serves as a standard in kinase activity assays and structural studies to elucidate molecular interactions. By providing a controlled, scalable source of the protein, recombinant MAP2K3 facilitates reproducible research into its physiological roles and therapeutic potential, bridging gaps between cellular signaling understanding and clinical applications.
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