| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP1LC3B2 |
| Uniprot No | A6NCE7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-120aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPSEKTFKQRRTFEQRVEDVRLIREQHPTK IPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYESEKDEDGFLYMVCASQETFG |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
MAP1LC3B2 (Microtubule-Associated Protein 1 Light Chain 3 Beta 2) is a key protein involved in autophagy, a conserved cellular process for degrading and recycling cytoplasmic components. It belongs to the LC3 protein family, which includes isoforms critical for autophagosome formation. MAP1LC3B2 is a splicing variant of MAP1LC3B, sharing structural homology with other LC3 family members but differing in specific sequence motifs that may influence its interaction partners or functional roles.
During autophagy, newly synthesized LC3B (proLC3) is processed by proteases to expose a C-terminal glycine, forming cytosolic LC3-I. Upon autophagy induction, LC3-I conjugates with phosphatidylethanolamine (PE) to generate lipidated LC3-II, which integrates into autophagosomal membranes. This lipidation step is essential for autophagosome expansion and cargo recruitment. MAP1LC3B2. like its canonical counterpart, is thought to participate in these membrane dynamics, serving as a marker for autophagic flux in experimental settings.
Recombinant MAP1LC3B2 protein is engineered for research applications, often expressed in bacterial or mammalian systems with tags (e.g., GFP, His-tag) for purification and detection. It enables mechanistic studies of autophagy regulation, particularly in disease contexts such as cancer, neurodegeneration, and infection, where autophagy plays dual roles in survival and death. Researchers use the recombinant protein in pull-down assays, immunoblotting, and microscopy to map protein interactions, quantify autophagic activity, or screen modulators of the pathway. Its role in selective autophagy subtypes (e.g., mitophagy, xenophagy) remains an active area of investigation, with potential implications for therapeutic targeting.
×
