| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAGOHB |
| Uniprot No | Q96A72 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAVASDF YLRYYVGHKG KFGHEFLEFE FRPDGKLRYA NNSNYKNDVM IRKEAYVHKS VMEELKRIID DSEITKEDDA LWPPPDRVGR QELEIVIGDE HISFTTSKIG SLIDVNQSKD PEGLRVFYYL VQDLKCLVFS LIGLHFKIKP I |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAGOHB Recombinant Protein**
MAGOHB (Mago Homolog B) is a conserved eukaryotic protein belonging to the MAGOH family, known for its critical role in mRNA processing and regulation. It is a core component of the exon junction complex (EJC), a multiprotein assembly deposited onto spliced mRNAs during nuclear pre-mRNA splicing. The EJC plays essential roles in post-transcriptional gene regulation, including mRNA export, localization, translation efficiency, and nonsense-mediated mRNA decay (NMD), a surveillance mechanism that degrades aberrant transcripts. MAGOHB interacts with other EJC components, such as EIF4A3 and RBM8A, forming a stable heterodimer with the latter to maintain EJC integrity.
The recombinant MAGOHB protein is engineered for research applications, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its recombinant form enables detailed biochemical studies, such as mapping protein-RNA interactions, dissecting EJC assembly mechanisms, and investigating its role in NMD. MAGOHB is also implicated in developmental processes and diseases; dysregulation of EJC components is linked to cancer, neurodevelopmental disorders, and viral infection responses. For instance, MAGOHB overexpression has been observed in certain cancers, suggesting potential roles in tumorigenesis or therapy resistance.
Structural studies using recombinant MAGOHB have clarified its binding interfaces with partner proteins and RNA, aiding drug discovery efforts targeting EJC-related pathologies. Additionally, MAGOHB's conserved nature across species makes it a valuable model for studying evolutionary aspects of mRNA metabolism. Researchers utilize recombinant MAGOHB to develop cellular and in vitro assays, advancing understanding of gene expression regulation and therapeutic interventions for EJC-associated diseases.
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