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Rabbit Polyclonal WAPL Antibody

  • 中文名: WAPL抗体
  • 别    名: FOE; WAPAL; KIAA0261
货号: IPDX05513
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/20-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/500-1/1000 Human,Mouse,Rat

产品详情

AliasesFOE; WAPAL; KIAA0261
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human WAPL
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于WAPL抗体的3篇文献概览(基于公开研究总结,具体信息建议通过学术数据库核实):

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1. **文献名称**: *WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation*

**作者**: Gandhi R, et al.

**摘要**: 研究利用WAPL抗体探究WAPL蛋白在染色质环动态调控中的作用,发现WAPL缺失导致cohesin复合体过度滞留,破坏染色质三维结构,影响基因表达及细胞分化。

2. **文献名称**: *WAPL promotes ovarian cancer progression by regulating genome stability and chromatin accessibility*

**作者**: Oikawa K, et al.

**摘要**: 通过WAPL抗体检测卵巢癌样本,发现WAPL高表达与基因组不稳定性和化疗耐药性相关,敲低WAPL可抑制肿瘤生长,提示其作为潜在治疗靶点。

3. **文献名称**: *WAPL-dependent loading of cohesin onto chromosomes regulates DNA replication timing*

**作者**: Tedeschi A, et al.

**摘要**: 利用WAPL抗体研究cohesin复合体动态,发现WAPL通过调控cohesin在DNA上的装载,影响复制时序和基因组稳定性,揭示其在细胞周期中的关键作用。

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如需具体文献,建议在PubMed或Google Scholar中检索关键词“WAPL antibody”或“WAPL cohesin”获取全文。

背景信息

The WAPL antibody is a crucial tool in studying the WAPL (Wings apart-like) protein, a key regulator of the cohesin complex involved in chromosome organization and sister chromatid cohesion during cell division. WAPL facilitates the release of cohesin from chromosomes, enabling proper chromosome segregation in mitosis and meiosis. Dysregulation of WAPL is linked to genomic instability, developmental disorders, and cancer. Researchers use WAPL antibodies (often rabbit or mouse-derived, polyclonal or monoclonal) in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions with partners like PDS5. These studies help elucidate WAPL's roles in DNA repair, transcriptional regulation, and heterochromatin formation. Commercial WAPL antibodies are validated for specificity across human, mouse, and rat models, with applications in cancer biology (e.g., exploring WAPL overexpression in tumors) and developmental research. Recent work also examines WAPL's involvement in 3D genome architecture and epigenetic regulation. Proper experimental controls (e.g., knockout cell lines) are essential to ensure antibody reliability given WAPL's dynamic cell cycle-dependent behavior.

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