| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HTX2; CFC1B; DTGA2; CRYPTIC |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CFC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CFC1抗体的示例参考文献(虚构示例，仅供格式参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *CFC1 Antibody Characterization in Left-Right Patterning Studies*
**作者**: Smith A, et al.
**摘要**: 本研究利用兔源多克隆CFC1抗体，通过免疫组化技术分析斑马鱼胚胎中CFC1蛋白的时空表达模式，揭示了其在左右轴形成初期Nodal信号通路中的关键定位。
2. **文献名称**: *Role of CFC1 in Congenital Heart Defects: Immunoblot Analysis*
**作者**: Chen L, et al.
**摘要**: 通过Western Blot和免疫荧光技术，作者使用人源CFC1单克隆抗体，证实CFC1基因突变患者心肌组织中CFC1蛋白表达显著降低，提示其与心脏房室管发育异常的关联。
3. **文献名称**: *CFC1 Antibody Blockade Disrupts Embryonic Stem Cell Differentiation*
**作者**: Tanaka K, et al.
**摘要**: 研究采用CFC1中和抗体阻断小鼠胚胎干细胞分化模型中的蛋白功能，证明CFC1对中胚层诱导及心脏前体细胞形成的调控作用。
4. **文献名称**: *Comparative Analysis of CFC1 Expression in Human Tissues Using Monoclonal Antibodies*
**作者**: Gupta R, et al.
**摘要**: 通过比较多种商业CFC1抗体的特异性，筛选出适用于石蜡切片的高效抗体，系统描述了CFC1在成人肝脏、心脏及肿瘤组织中的差异表达。
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**注意**：以上为模拟示例，实际文献需查阅PubMed、Web of Science等平台(关键词：CFC1 antibody, Cryptic protein, EGF-CFC family)。真实研究可能涉及发育生物学、疾病机制或抗体应用技术优化。
CFC1 (Cripto-1/FRL-1/Cryptic family protein 1) antibodies target a member of the EGF-CFC protein family, which plays critical roles in embryonic development, particularly in the Nodal signaling pathway. CFC1 acts as a co-receptor for Nodal, a TGF-β superfamily ligand, facilitating its binding to activin receptors and activating downstream Smad2/3 signaling. This pathway is essential for establishing left-right asymmetry, gastrulation, and organogenesis in vertebrates. Dysregulation of CFC1 is linked to congenital malformations, including heterotaxy syndrome, and cancer progression due to its oncogenic properties in certain contexts.
CFC1 antibodies are vital tools for studying protein expression, localization, and function in developmental biology and disease models. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess CFC1 levels in tissues or cell lines. Additionally, neutralizing CFC1 antibodies have therapeutic potential, as blocking CFC1-Nodal interactions may inhibit tumor growth or metastasis in cancers where CFC1 is overexpressed (e.g., breast, colorectal). These antibodies also aid in elucidating molecular mechanisms of developmental disorders, offering insights into potential diagnostic or therapeutic strategies. Research continues to explore their applications in regenerative medicine and targeted cancer therapies.
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