| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK8 |
| Uniprot No | P45983 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4-422aa |
| 氨基酸序列 | SKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGSQHPSSSSSVNDVSSMSTDPTLASDTDSSLEAAAGP |
| 预测分子量 | 51.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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MAPK8 (Mitogen-Activated Protein Kinase 8), also known as JNK1 (c-Jun N-terminal kinase 1), is a member of the serine/threonine protein kinase family within the MAPK signaling pathway. It plays a critical role in regulating cellular responses to stress signals, cytokines, and growth factors, influencing processes such as apoptosis, proliferation, differentiation, and inflammatory responses. The MAPK8 gene encodes a protein that is activated through dual phosphorylation by upstream MAP2Ks (e.g., MKK4/MKK7) in response to extracellular stimuli, including oxidative stress, DNA damage, or inflammatory signals. Once activated, MAPK8 translocates to the nucleus, where it phosphorylates transcription factors like c-Jun, ATF2. and p53. modulating gene expression to adapt to environmental changes.
Recombinant MAPK8 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce highly purified, functional kinase for research and drug development. It typically retains enzymatic activity and is used in vitro to study kinase-substrate interactions, screen inhibitors, or elucidate signaling mechanisms. Researchers utilize tagged versions (e.g., His-tag, GST-tag) for ease of purification and detection. Its recombinant form is vital for dissecting JNK pathway dysregulation linked to diseases like cancer, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes. For example, aberrant MAPK8 activity is implicated in tumor progression and chemotherapy resistance, making it a therapeutic target. Additionally, recombinant MAPK8 aids in developing kinase-specific assays and personalized medicine strategies, bridging molecular insights with clinical applications.
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