| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOX4; HOX4E; HOX5.4 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HOXD8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HOXD8抗体的3篇参考文献示例(文献为示例性内容，实际引用需核实)：
1. **文献名称**: "HOXD8 regulates mesenchymal stem cell fate in skeletal development through epigenetic modulation"
**作者**: Li, Y., et al.
**摘要**: 研究利用HOXD8特异性抗体通过ChIP-seq分析，发现HOXD8通过调控Wnt信号通路关键基因的H3K27me3修饰，影响间充质干细胞的成骨分化，揭示其在骨骼发育中的表观遗传调控机制。
2. **文献名称**: "Aberrant HOXD8 expression promotes tumor invasion in colorectal cancer"
**作者**: Smith, J.R., & Wang, H.
**摘要**: 通过免疫组化(HOXD8抗体)和RNA干扰实验，证实HOXD8在结直肠癌中高表达，其通过激活MMP9促进肿瘤细胞侵袭，提示HOXD8可作为潜在治疗靶点。
3. **文献名称**: "HOXD8 antibody-based profiling reveals spatial expression patterns during mouse embryogenesis"
**作者**: Garcia-Fernàndez, J., & Holland, P.W.
**摘要**: 使用HOXD8单克隆抗体对小鼠胚胎进行免疫荧光染色，发现其在肢芽和神经管发育中的梯度表达，为HOX基因在体轴形成中的作用提供形态学证据。
注：以上文献信息为模拟示例，实际研究需通过PubMed/Google Scholar等平台以“HOXD8 antibody”为关键词检索最新文献。
The HOXD8 antibody is a research tool designed to detect the HOXD8 protein, a member of the homeobox (HOX) gene family. HOX genes encode transcription factors critical for regulating embryonic development, particularly in establishing body patterning along the anterior-posterior axis. The HOXD8 gene, located on chromosome 2 (2q31.1), is part of the HOXD cluster, which plays roles in limb development, neural tube formation, and urogenital system differentiation. HOXD8 protein is characterized by a conserved 60-amino acid DNA-binding homeodomain, enabling sequence-specific interactions to regulate downstream gene expression.
HOXD8 antibodies are widely used in developmental biology and cancer research. They facilitate the identification of HOXD8 expression patterns via techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence. Studies have linked HOXD8 dysregulation to pathological conditions, including cancers (e.g., colorectal, prostate) and developmental disorders. For instance, reduced HOXD8 expression has been associated with tumor metastasis, suggesting its role as a potential tumor suppressor. Conversely, overexpression in certain contexts may influence cellular proliferation or differentiation.
Despite their utility, HOXD8 antibodies require rigorous validation due to potential cross-reactivity with other HOX family members. Researchers prioritize antibodies with high specificity, often verified through knockout controls or peptide-blocking assays. Ongoing investigations aim to clarify HOXD8's mechanistic roles in development and disease, highlighting its importance as a biomarker or therapeutic target.
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