Rabbit Polyclonal E2F8 Antibody

The E2F8 antibody is a crucial tool for studying the E2F transcription factor family, particularly E2F8. which belongs to the atypical E2F subgroup (E2F7 and E2F8). Unlike canonical E2Fs (E2F1-E2F6) that primarily regulate cell cycle progression by activating or repressing target genes, atypical E2Fs act as transcriptional repressors. E2F8 plays a dual role in maintaining genomic stability by modulating G1/S and G2/M cell cycle checkpoints and participating in DNA damage response pathways. It is essential for embryonic development, as evidenced by studies in knockout models showing lethal phenotypes. In cancer, E2F8 exhibits context-dependent functions: it may promote tumorigenesis in certain cancers (e.g., liver, breast) by enhancing proliferation and survival, while acting as a tumor suppressor in others. The E2F8 antibody enables researchers to detect protein expression levels, subcellular localization, and interactions via techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation. Its specificity is critical due to structural similarities among E2F family members, and validation often includes knockout controls. Recent studies highlight E2F8's potential as a therapeutic target, particularly in cancers with dysregulated cell cycle pathways, driving interest in antibodies that reliably capture its complex roles in health and disease.