| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; NURF140 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SMARCA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SMARCA1抗体的代表性文献(内容基于模拟生成，仅供参考)：
1. **"Generation and characterization of a monoclonal antibody specific for SMARCA1 in chromatin remodeling studies"**
- **作者**: Li, X., et al.
- **摘要**: 本研究开发了一种针对SMARCA1蛋白的单克隆抗体，验证了其在免疫印迹(Western blot)和免疫荧光中的特异性，并用于探究SMARCA1在HeLa细胞染色质重塑复合体中的定位及功能。
2. **"SMARCA1 regulates pluripotency exit in embryonic stem cells via histone modification"**
- **作者**: Garcia, S.M., et al.
- **摘要**: 通过使用商业化SMARCA1抗体(货号AB123.Sigma)，研究发现SMARCA1通过调控H3K9ac修饰介导胚胎干细胞多能性退出，抗体在ChIP-seq和免疫共沉淀(Co-IP)中验证有效。
3. **"Antibody-based profiling of SWI/SNF complex subunits in lung cancer"**
- **作者**: Patel, R., & Kim, J.
- **摘要**: 文章比较了多种SWI/SNF亚基抗体(包括SMARCA1)在肺癌组织中的表达差异，发现SMARCA1抗体(克隆号3B7)可有效用于免疫组化(IHC)检测，并与患者预后相关。
注：以上文献为示例，实际引用时请以真实数据库(如PubMed、Web of Science)检索结果为准。
SMARCA1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1) is a helicase protein encoded by the *SMARCA1* gene, also known as *SNF2L* or *ARAB1*. It is a core component of the ATP-dependent chromatin-remodeling complex ISWI (imitation switch), which regulates gene expression by altering nucleosome positioning and chromatin structure. SMARCA1 plays critical roles in transcriptional activation/repression, DNA repair, and cellular differentiation. It is particularly important in neurodevelopment, stem cell maintenance, and hematopoiesis.
Antibodies targeting SMARCA1 are essential tools for studying its function and expression in these biological processes. They are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry to detect protein levels, localization, and DNA-protein interactions. Commercially available SMARCA1 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and validated for specificity in human, mouse, or rat samples.
Dysregulation of SMARCA1 is implicated in cancers (e.g., leukemia) and neurodevelopmental disorders, driving research into its mechanistic roles. Reliable antibodies help identify SMARCA1’s interaction partners, downstream targets, and epigenetic regulatory pathways. However, users must verify antibody specificity due to potential cross-reactivity with homologous proteins like SMARCA4 (BRG1), another SWI/SNF family member. Recent studies also explore SMARCA1’s role in aging and metabolic diseases, expanding its therapeutic relevance.
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