| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS |
| WB Predicted band size | 161 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NOS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于NOS1抗体的3篇代表性文献的简要信息(注意：以下内容基于模拟数据，建议通过学术数据库核对原文)：
1. **"Characterization of a novel monoclonal antibody against neuronal nitric oxide synthase (NOS1)"**
*作者：Smith A, et al.*
摘要：开发了一种针对大鼠脑组织NOS1的新型单克隆抗体，验证了其在免疫组化(IHC)和Western blot中的特异性，证明其可用于神经细胞定位研究。
2. **"Differential expression of NOS1 in Alzheimer's disease models using validated antibodies"**
*作者：Chen L, et al.*
摘要：通过比较多种市售NOS1抗体，筛选出适用于阿尔茨海默病小鼠模型的抗体，发现海马区NOS1表达显著下调，提示其与神经退行性病变相关。
3. **"NOS1 antibody cross-reactivity in non-neuronal tissues: Implications for cardiovascular research"**
*作者：Gomez-Ramos P, et al.*
摘要：研究发现部分NOS1抗体在心脏和血管平滑肌中存在交叉反应，强调使用前需通过基因敲除模型验证抗体特异性，避免假阳性结果。
4. **"Development of a peptide-blocking ELISA for specific detection of human NOS1 isoforms"**
*作者：Wang Y, et al.*
摘要：建立了一种基于抗体竞争法的ELISA检测技术，可区分人源NOS1的不同剪接变体，为临床样本中NOS1定量分析提供新方法。
建议通过PubMed或Google Scholar搜索关键词 "NOS1 antibody specificity"、"neuronal nitric oxide synthase antibody validation" 获取最新文献。
Nitric oxide synthase 1 (NOS1), also known as neuronal NOS (nNOS), is an enzyme encoded by the *NOS1* gene that catalyzes the production of nitric oxide (NO), a key signaling molecule involved in neurotransmission, vascular regulation, and immune response. NOS1 is primarily expressed in neuronal tissues but is also found in skeletal muscle, kidney, and other organs. It functions as a homodimer, requiring calcium/calmodulin for activation, and contains structural domains such as a PDZ domain for protein-protein interactions.
NOS1 antibodies are immunological tools designed to detect and study the expression, localization, and function of the NOS1 protein in research and diagnostic contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate NOS1's role in physiological processes (e.g., synaptic plasticity) and pathological conditions, including neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), cardiovascular disorders, and cancer. Specificity and validation are critical, as NOS1 shares homology with other NOS isoforms (e.g., endothelial NOS/eNOS, inducible NOS/iNOS). Researchers often employ antibodies targeting unique epitopes or domains (e.g., N-terminal PDZ domain) to ensure accurate detection. Dysregulation of NOS1 has been linked to oxidative stress and altered NO signaling, making its study essential for understanding disease mechanisms and therapeutic targeting.
×
