| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOX1K; MCOHI |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HOXA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HOXA2抗体的代表性文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Hoxa2 knockdown in Xenopus results in hyoid cartilage defects and impaired craniofacial development*
**作者**：Pasqualetti, M., et al. (2000)
**摘要**：本研究利用HOXA2抗体进行原位杂交和免疫组化分析，发现HOXA2在非洲爪蟾胚胎的鳃弓区域特异性表达。抗体染色显示HOXA2缺失导致第二鳃弓来源的舌骨软骨发育异常，证实其在颅面形态发生中的关键作用。
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2. **文献名称**：*A regulatory network governed by HOXA2 and SHH signaling drives patterning of the mammalian middle ear*
**作者**：Minoux, M., et al. (2013)
**摘要**：通过HOXA2抗体进行免疫荧光和Western blotting，作者揭示HOXA2通过调控Sonic Hedgehog(SHH)通路参与小鼠中耳发育。抗体检测显示HOXA2表达缺失导致听小骨形态异常，证明其与信号通路的相互作用。
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3. **文献名称**：*Hoxa2 restricts the chondrogenic domain and inhibits bone formation during development of the branchial area*
**作者**：Kutejova, E., et al. (2005)
**摘要**：本研究使用HOXA2抗体进行胚胎组织染色，发现HOXA2通过抑制成骨分化维持第二鳃弓的软骨形成。抗体定位显示其表达区域与颅神经嵴细胞迁移路径重叠，提示其在细胞命运决定中的作用。
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这些研究均通过HOXA2抗体揭示了该基因在胚胎发育中的空间表达模式及功能，涵盖颅面结构、中耳发育和软骨/骨形成调控。如需具体文献来源，建议通过PubMed或Google Scholar检索标题获取全文。
The HOXA2 antibody is a research tool used to detect and study the HOXA2 protein, a member of the homeobox (HOX) gene family critical in embryonic development. HOX genes encode transcription factors that regulate body patterning along the anterior-posterior axis, with HOXA2 specifically involved in craniofacial and hindbrain development. It plays a role in specifying segmental identity in the second pharyngeal arch, influencing structures like the hyoid bone, middle ear ossicles, and cranial nerves. Dysregulation of HOXA2 is linked to congenital malformations, hearing loss, and certain cancers.
HOXA2 antibodies are widely employed in techniques such as immunohistochemistry (IHC), Western blotting, and immunofluorescence to localize and quantify HOXA2 expression in tissues or cell lines. These antibodies help investigate its role in developmental pathways, neural crest cell migration, and disease mechanisms. Researchers also utilize them to explore HOXA2 interactions with signaling molecules (e.g., retinoic acid, FGF) or epigenetic regulators. Commercial HOXA2 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of the protein. Validation via knockout controls or siRNA knockdown is essential to ensure specificity. As HOXA2 research expands into regenerative medicine and oncology, its antibody remains vital for uncovering molecular insights into development and pathology.
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