| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IFCR; MGA1; gp280 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CUBN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CUBN(cubilin)抗体的参考文献，供参考：
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1. **文献名称**: *Cubilin is essential for albumin reabsorption in the renal proximal tubule*
**作者**: Amsellem S, et al.
**摘要**: 该研究通过CUBN抗体免疫组化分析，证实cubilin蛋白在肾近端小管上皮细胞中的表达，并揭示其在白蛋白重吸收中的关键作用。基因敲除小鼠模型显示CUBN缺失导致蛋白尿，提示其与慢性肾病相关。
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2. **文献名称**: *Imerslund-Gräsbeck syndrome-associated mutations in the cubilin ligand-binding domain impair protein stability and trafficking*
**作者**: Kristiansen M, et al.
**摘要**: 研究利用CUBN抗体进行免疫印迹和免疫荧光实验，发现遗传性维生素B12吸收障碍(IGS)患者的CUBN基因突变导致蛋白结构异常，影响其在肠上皮细胞的定位及功能。
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3. **文献名称**: *Megalin and cubilin in proximal tubule protein reabsorption: from experimental models to human disease*
**作者**: Christensen EI, Birn H.
**摘要**: 综述性研究总结了CUBN抗体在肾脏疾病研究中的应用，阐明cubilin与megalin形成的复合物介导肾小管对低分子量蛋白的摄取，并探讨其在糖尿病肾病等疾病中的病理机制。
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如需更多文献，可进一步限定研究领域(如肠道吸收、癌症等)。
**Background of CUBN Antibody**
Cubilin (CUBN), encoded by the *CUBN* gene, is a large transmembrane glycoprotein functioning as a multiligand endocytic receptor. Primarily expressed in polarized epithelia, such as the renal proximal tubules and small intestine, it plays critical roles in nutrient uptake (e.g., vitamin B12) and protein reabsorption. CUBN lacks transmembrane domains but anchors to cell membranes via interactions with megalin (LRP2) and the transmembrane protein AMN (amnionless), forming the cubam complex. This complex mediates ligand internalization via clathrin-coated pits.
CUBN antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. Dysregulation of CUBN is linked to Imerslund-Gräsbeck syndrome (IGS), a rare genetic disorder characterized by vitamin B12 deficiency and proteinuria, as well as chronic kidney disease (CKD) due to impaired protein reabsorption. In research, CUBN antibodies enable immunohistochemical detection in tissues, Western blot analysis, and investigation of renal and intestinal pathologies. They also aid in exploring CUBN’s role in drug transport, metabolic disorders, and cancer, where altered expression may influence disease progression. Species-specific CUBN antibodies (e.g., human, mouse) are widely utilized to dissect molecular mechanisms in model systems.
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