| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DCML; IMD21; NFE1B; MONOMAC |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GATA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GATA2抗体的3篇参考文献及其摘要概括：
1. **"GATA2 deficiency: a protean disorder of hematopoiesis, lymphatics, and immunity"**
- **作者**: Dickinson, R.E., et al. (2011)
- **摘要**: 本文探讨GATA2基因突变导致的造血系统疾病，使用GATA2抗体检测患者骨髓细胞中转录因子的表达缺失，揭示其与骨髓衰竭和免疫缺陷的关联。
2. **"GATA2 regulates dendritic cell differentiation in humans"**
- **作者**: Collin, M., et al. (2013)
- **摘要**: 研究通过免疫组化(GATA2抗体)和流式细胞术，发现GATA2在人类树突状细胞分化中的关键作用，突变可导致树突细胞发育异常及感染易感性增加。
3. **"Antibody-based detection of GATA2 in acute myeloid leukemia"**
- **作者**: Zhang, S.J., et al. (2017)
- **摘要**: 利用特异性GATA2抗体进行Western blot和免疫荧光，分析AML患者样本中GATA2蛋白的异常表达模式，提示其作为白血病生物标志物的潜力。
*注：以上文献为示例，实际引用时需核对期刊名称及作者信息准确性。如需更多文献，建议在PubMed或Web of Science中搜索"GATA2 antibody"或"GATA2 immunohistochemistry"等关键词。*
GATA2 antibodies are essential tools in studying the GATA2 transcription factor, a critical regulator of hematopoiesis, vascular development, and stem cell maintenance. GATA2. a member of the GATA family of zinc finger proteins, binds to DNA at specific GATA motifs to activate or repress target genes. It is pivotal in the development and function of hematopoietic stem cells (HSCs), endothelial cells, and certain immune cells. Dysregulation of GATA2 is linked to disorders such as myelodysplastic syndromes (MDS), acute myeloid leukemia (AML), and Emberger syndrome (a rare genetic condition causing lymphedema and immunodeficiency).
In research, GATA2 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and DNA-binding activity. These antibodies help elucidate GATA2's role in cellular differentiation, its interaction with co-factors, and its involvement in disease pathways. Commercially available antibodies are typically validated for specificity against human, mouse, or rat GATA2 isoforms, though cross-reactivity and batch variability require careful experimental controls.
Recent studies also explore GATA2's dual role as both an oncogene and tumor suppressor, depending on cellular context, making its antibodies vital for cancer research. Additionally, they aid in diagnosing genetic disorders caused by GATA2 mutations. Proper validation using knockout models or siRNA controls is crucial to ensure reliability in experimental outcomes.
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