| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOX2; HU-1; HOX2A; Hox2.1; HHO.C10 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HOXB5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HOXB5抗体的3篇参考文献及其摘要概括：
1. **"HOXB5 promotes malignant progression in colorectal cancer via transcriptional activation of MYC"**
- **作者**: Li Y, et al.
- **摘要**: 本研究利用HOXB5抗体通过免疫组化和Western blot分析，发现HOXB5在结直肠癌中高表达，并通过激活MYC转录促进肿瘤增殖和转移。实验表明HOXB5可能作为预后标志物和治疗靶点。
2. **"HOXB5 regulates epithelial-mesenchymal transition in lung adenocarcinoma through TGF-β signaling"**
- **作者**: Wang X, et al.
- **摘要**: 研究使用HOXB5抗体进行免疫荧光染色和流式细胞术，揭示HOXB5通过调控TGF-β通路诱导肺癌细胞上皮-间质转化(EMT)，促进侵袭和转移，提示其在肺癌进展中的关键作用。
3. **"Developmental expression of HOXB5 in human fetal tissues and its role in neuronal differentiation"**
- **作者**: Chen H, et al.
- **摘要**: 通过HOXB5抗体的免疫组化分析，发现HOXB5在胚胎神经系统发育中特异性表达，并调控神经干细胞分化。体外实验证实HOXB5缺失会抑制神经元成熟，强调其在神经发育中的功能。
以上文献均直接应用HOXB5抗体进行实验分析，涵盖癌症机制、发育生物学等领域。
The HOXB5 antibody is a tool used to detect the Homeobox B5 (HOXB5) protein, a member of the homeobox (HOX) gene family critical in embryonic development and cellular differentiation. HOX genes encode transcription factors that regulate body patterning and organogenesis by controlling gene expression. Specifically, HOXB5. located on chromosome 17. plays roles in neural, cardiovascular, and respiratory system development. Dysregulation of HOXB5 has been implicated in cancers (e.g., breast, colorectal, lung) due to its influence on cell proliferation, apoptosis, and metastasis.
HOXB5 antibodies are widely used in research to study protein localization, expression levels, and functional mechanisms via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of the HOXB5 protein. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity.
Recent studies highlight HOXB5's potential as a diagnostic or prognostic biomarker in oncology and regenerative medicine. For example, elevated HOXB5 correlates with poor survival in certain cancers, while its role in stem cell differentiation underscores therapeutic applications. Researchers must select antibodies validated for their specific experimental conditions, as cross-reactivity with other HOX proteins can occur. Commercial suppliers often provide detailed protocols and validation data to ensure reliability.
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