| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAT1A |
| Uniprot No | Q00266 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF |
| 预测分子量 | 47.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAT1A Recombinant Protein**
Methionine adenosyltransferase 1A (MAT1A) is a key enzyme encoded by the *MAT1A* gene, primarily expressed in the liver. It catalyzes the synthesis of S-adenosylmethionine (SAMe), the principal methyl donor in cellular methylation reactions and a precursor for polyamines and glutathione. SAMe is critical for maintaining hepatic function, lipid metabolism, and redox balance. MAT1A deficiency or dysregulation is linked to liver pathologies, including hepatocellular carcinoma (HCC), cirrhosis, and non-alcoholic fatty liver disease (NAFLD), as well as systemic conditions like atherosclerosis.
Recombinant MAT1A protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to enable functional studies and therapeutic exploration. Its production involves cloning the *MAT1A* gene into expression vectors, followed by purification via affinity chromatography. The recombinant protein retains enzymatic activity, allowing researchers to investigate SAMe metabolism, methylation-dependent pathways, and disease mechanisms.
Studies using MAT1A recombinant protein have highlighted its role in suppressing liver cancer progression by restoring SAMe levels, which regulate DNA methylation, apoptosis, and tumor suppressor genes. It also modulates lipid homeostasis, making it a potential target for metabolic disorders. Additionally, recombinant MAT1A serves as a tool for drug screening, aiming to develop agents that enhance SAMe synthesis or mimic its effects.
Overall, MAT1A recombinant protein is vital for deciphering the enzyme’s biological roles and its therapeutic potential in liver-related and metabolic diseases. Ongoing research focuses on optimizing its stability, delivery, and efficacy in preclinical models.
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