| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPRE3 |
| Uniprot No | Q15691 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-281aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
**Background of MAPRE3 Recombinant Protein**
MAPRE3 (Microtubule-Associated Protein RP/EB Family Member 3), also known as end-binding protein 3 (EB3), is a member of the EB family of microtubule-associated proteins (MAPs) that play critical roles in regulating microtubule dynamics. EB proteins, including EB1. EB2. and EB3. are conserved across eukaryotes and are essential for stabilizing microtubules, guiding their growth, and mediating interactions with cellular components involved in mitosis, intracellular transport, and cell polarity.
MAPRE3 specifically binds to the growing plus-ends of microtubules, a process termed "microtubule tip tracking," and facilitates the recruitment of other +TIPs (+end-tracking proteins) to coordinate microtubule interactions with cellular structures. Structurally, it contains a calponin homology (CH) domain for microtubule binding and an EB homology domain for protein-protein interactions. Dysregulation of MAPRE3 has been linked to neurological disorders, cancer metastasis, and developmental defects, underscoring its importance in cellular homeostasis.
Recombinant MAPRE3 protein is produced using expression systems (e.g., *E. coli* or mammalian cells*) engineered to overexpress the protein, followed by purification via affinity chromatography. This engineered protein retains native functionality, enabling studies on microtubule dynamics, protein interaction networks, and mechanisms underlying diseases. Researchers utilize MAPRE3 recombinant protein in *in vitro* assays, live-cell imaging, and structural studies to dissect its role in cellular processes or screen for therapeutic agents targeting microtubule-related pathologies. Its availability accelerates biomedical research bridging molecular biology and clinical applications.
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