| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPRE2 |
| Uniprot No | Q15555 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MPGPTQTLSP NGENNNDIIQ DNNGTIIPFR KHTVRGERSY SWGMAVNVYS TSITQETMSR HDIIAWVNDI VSLNYTKVEQ LCSGAAYCQF MDMLFPGCIS LKKVKFQAKL EHEYIHNFKL LQASFKRMNV DKVIPVEKLV KGRFQDNLDF IQWFKKFYDA NYDGKEYDPV EARQGQDAIP PPDPGEQIFN LPKKSHHANS PTAGAAKSSP AAKPGSTPSR PSSAKRASSS GSASKSDKDL ETQVIQLNEQ VHSLKLALEG VEKERDFYFG KLREIELLCQ EHGQENDDLV QRLMDILYAS EEHEGHTEEP EAEEQAHEQQ PPQQEEY |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
**Background of MAPRE2 Recombinant Protein**
MAPRE2 (Microtubule-Associated Protein RP/EB Family Member 2), also known as EB2. is a member of the end-binding (EB) protein family, which plays a critical role in regulating microtubule dynamics. Microtubules are essential cytoskeletal components involved in cell division, intracellular transport, and maintenance of cell shape. EB proteins, including MAPRE2. stabilize growing microtubule plus-ends by binding to GTP-tubulin caps, thereby modulating microtubule polymerization, interactions with cellular components, and recruitment of other microtubule-associated proteins.
Structurally, MAPRE2 contains a conserved N-terminal calponin homology (CH) domain for microtubule binding and a C-terminal region that mediates interactions with other proteins, such as +TIPs (microtubule plus-end tracking proteins). Unlike its homologs EB1 (MAPRE1) and EB3 (MAPRE3), MAPRE2 exhibits distinct expression patterns and regulatory roles. It is highly expressed in tissues like the brain and is implicated in neuronal development, cell migration, and mitotic spindle orientation. Dysregulation of MAPRE2 has been linked to neurological disorders and cancers, highlighting its biomedical relevance.
Recombinant MAPRE2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. The recombinant form often includes affinity tags (e.g., His-tag, GST) for purification and detection. Researchers utilize this protein to investigate microtubule dynamics, protein-protein interactions, and signaling pathways in vitro or in cell-based assays. It also serves as a tool for drug screening targeting microtubule-related diseases. Quality control involves verifying purity (via SDS-PAGE), structural integrity (mass spectrometry), and functional activity (e.g., microtubule-binding assays).
Overall, recombinant MAPRE2 is a vital reagent for dissecting microtubule biology and exploring therapeutic strategies in cancer and neurodegenerative conditions.
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