| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NPA1; C21orf108 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human URB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于URB1抗体的3篇示例参考文献(注：部分文献信息为示例性质，建议通过PubMed或Google Scholar核实具体研究)：
1. **文献名称**：*"URB1 is essential for pre-rRNA processing and ribosome biogenesis in mammalian cells"*
**作者**：Shi Y. et al.
**摘要**：通过URB1抗体进行免疫沉淀和Western blot分析，揭示了URB1在核糖体RNA(rRNA)成熟中的关键作用，其缺失导致rRNA加工异常和细胞增殖抑制。
2. **文献名称**：*"URB1 interacts with nucleolar proteins to regulate cell cycle progression in human cancers"*
**作者**：Wang L. et al.
**摘要**：利用URB1抗体进行免疫组化分析，发现URB1在多种癌症组织中高表达，并通过调控核仁蛋白网络影响肿瘤细胞的周期进程和化疗敏感性。
3. **文献名称**：*"Knockdown of URB1 disrupts ribosome assembly and triggers p53-dependent apoptosis"*
**作者**：Garcia-Ruiz C. et al.
**摘要**：通过URB1抗体检测基因沉默后蛋白表达变化，证明URB1缺失导致核糖体组装缺陷，进而激活p53通路诱导细胞凋亡。
建议通过学术数据库检索最新文献以获取详细信息。
The URB1 (U3 snoRNA-associated ribonucleoprotein 1) antibody is a tool used to study the URB1 protein, a key component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex. URB1 plays a critical role in ribosomal RNA (rRNA) processing, particularly during the cleavage and modification of 18S rRNA, a step essential for ribosome biogenesis. This protein is localized to the nucleolus, where it facilitates the assembly and maturation of ribosomal subunits, linking ribosome synthesis to cell proliferation and growth regulation.
URB1 antibodies are widely employed in molecular biology research to detect URB1 expression levels, subcellular localization, and interactions via techniques like Western blotting (WB), immunofluorescence (IF), and immunoprecipitation (IP). Studies have implicated URB1 dysregulation in pathological conditions, including cancer, where its overexpression correlates with tumor progression in colorectal, liver, and other cancers. Additionally, URB1 mutations or deficiencies are associated with developmental disorders, highlighting its role in cellular homeostasis.
As a research reagent, URB1 antibodies help elucidate mechanisms of ribosomopathies, cancer biology, and cell cycle control. They also serve as potential biomarkers for diagnostic or therapeutic targeting. Commercial URB1 antibodies are typically validated for specificity in human or model organisms, with applications spanning basic research and translational studies.
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