Mouse Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

货号: IPD-E04564m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠粒细胞集落刺激因子(G-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的CSF3含量。CSF3，即集落刺激因子3，是IL-6超家族的一员，主要在骨髓造血细胞中表达，通过与其特异性受体结合，激活JAK/STAT、MAPK等信号通路，促进粒细胞增殖、分化和存活，参与免疫系统发育和功能，尤其对中性粒细胞生成和活化有重要作用。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。该产品可广泛应用于免疫学、炎症机制研究或药物开发等领域，例如评估实验动物模型中G-CSF的动态变化、研究炎症性疾病相关通路或筛选调节中性粒细胞生成的化合物，为科研人员探索G-CSF在生理调控及病理状态下的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Csf3 ELISA kit; CsfgGranulocyte colony-stimulating factor ELISA kit; G-CSF ELISA kit
缩写：
CSF3
Uniprot No.：
P09920
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma,tissue homogenates
检测范围：
7.8 pg/mL-500 pg/mL
灵敏度：
1.95 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)62.69362.80162.47762.58562.15562.908SD0.0330.0280.0300.0370.0420.039CV(%)4.3423.6793.9584.8755.5635.118

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	87
	Range %	85-89
1:2	Average %	102
	Range %	99-106
1:4	Average %	98
	Range %	96-102
1:8	Average %	92
	Range %	90-94

回收率：

The recovery of mouse G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
500	2.897	2.964	2.931	2.785
250	2.089	2.103	2.096	1.950
125	1.311	1.330	1.321	1.175
62.5	0.724	0.711	0.718	0.572
31.2	0.479	0.454	0.467	0.321
15.6	0.319	0.342	0.331	0.185
7.8	0.234	0.244	0.239	0.093
0	0.141	0.151	0.146