| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CYP11B2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CYP11B2抗体的代表性文献摘要：
1. **"Production and characterization of monoclonal antibodies against human CYP11B2 and CYP11B1"**
*作者: Ye P. et al. (2007)*
摘要：该研究开发了针对人CYP11B2(醛固酮合成酶)和CYP11B1(11β-羟化酶)的单克隆抗体，验证了其特异性，可区分两者在肾上腺组织中的表达差异，为原发性醛固酮增多症的病理研究提供工具。
2. **"Immunohistochemistry of aldosterone synthase leads to breakthrough in understanding of adrenal pathophysiology"**
*作者: Gomez-Sanchez CE. et al. (2014)*
摘要：通过优化CYP11B2抗体的免疫组化方法，揭示了肾上腺腺瘤与增生组织中醛固酮合成的空间分布特征，推动了对原发性醛固酮增多症分子机制的理解。
3. **"CYP11B2 expression in normal and pathological adrenals"**
*作者: Nakamura Y. et al. (2011)*
摘要：利用特异性抗体分析CYP11B2在正常肾上腺及肾上腺肿瘤中的表达模式，发现其在醛固酮产生腺瘤中高表达，而在皮质腺瘤中缺失，支持其作为诊断生物标志物的潜力。
4. **"Role of CYP11B2 in cardiac fibrosis"**
*作者: Lian X. et al. (2016)*
摘要：通过免疫荧光染色结合CYP11B2抗体，证实肾上腺外(心脏)局部醛固酮合成酶表达与心肌纤维化的相关性，为心血管疾病治疗提供新靶点。
注：以上文献为示例性质，实际引用时需核对具体论文信息及数据库(如PubMed)的完整条目。
The CYP11B2 antibody targets the enzyme aldosterone synthase, encoded by the CYP11B2 gene, which plays a critical role in the renin-angiotensin-aldosterone system (RAAS). CYP11B2 is a mitochondrial cytochrome P450 enzyme predominantly expressed in the zona glomerulosa of the adrenal cortex. It catalyzes the final steps of aldosterone biosynthesis, converting 11-deoxycorticosterone to corticosterone and then to aldosterone via hydroxylation and oxidation. Aldosterone regulates blood pressure and electrolyte balance by promoting sodium retention and potassium excretion in the kidneys.
CYP11B2 antibodies are widely used in research to study aldosterone-producing adenomas (APAs), primary aldosteronism, and adrenal disorders. These antibodies help localize CYP11B2 expression in tissues, distinguishing it from the closely related enzyme CYP11B1 (11β-hydroxylase), which is involved in cortisol synthesis. Specific detection of CYP11B2 is crucial for understanding pathological conditions like hypertension and adrenal hyperplasia, where dysregulated aldosterone production occurs.
In diagnostic and experimental settings, CYP11B2 antibodies enable immunohistochemical staining, Western blotting, and ELISA to assess enzyme activity or expression levels. Their specificity aids in identifying somatic mutations (e.g., KCNJ5 variants) in APAs and evaluating therapeutic targets for aldosterone-related diseases. Research using these antibodies continues to clarify mechanisms of aldosterone regulation and its role in cardiovascular and renal pathologies.
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