首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胱抑素B(CSTB/CST6/STFB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、唾液、组织匀浆、细胞裂解物样本中的CSTB含量。CSTB即半胱氨酸蛋白酶抑制剂B，是一种内源性的半胱氨酸蛋白酶抑制剂。其突变与进行性肌阵挛性癫痫1型相关。研究机制上，它主要通过抑制半胱氨酸蛋白酶活性，维持细胞内蛋白代谢平衡，在神经系统的稳定等方面发挥重要作用。试剂盒检测范围为0.313 ng/mL-20 ng/mL，灵敏度为0.233 ng/mL。该产品适用于基础科研中探索胱抑素B在神经退行性疾病模型、肿瘤微环境调控、炎症信号通路中的表达规律，或作为生物标志物在体液样本中的浓度变化研究，为疾病机制解析及药物开发提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CHROW21 ELISA Kit; CPI B ELISA Kit; CPI-B ELISA Kit; CST 6 ELISA Kit; CST6 ELISA Kit; CSTB ELISA Kit; Cystatin B (stefin B) ELISA Kit; Cystatin B ELISA Kit; Cystatin-B ELISA Kit; CYTB ELISA Kit; CYTB_HUMAN ELISA Kit; EPM1 ELISA Kit; EPM1A ELISA Kit; Liver thiol proteinase inhibitor ELISA Kit; PME ELISA Kit; Stefin-B ELISA Kit; STF B ELISA Kit; STFB ELISA Kit; ULD ELISA Kit
缩写：CSTB
Uniprot No.：P04080
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, saliva, tissue homogenates, cell lysates
检测范围：0.313 ng/mL-20 ng/mL
灵敏度：0.233 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CSTB/CST6/STFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 101-106 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 88-97 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 91-104 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-94 | ||||||
回收率：
| The recovery of human CSTB/CST6/STFB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 99-108 | |||||
| EDTA plasma (n=4) | 98 | 93-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.4112.5882.5002.340 101.7021.8111.7571.597 51.1661.1631.1651.005 2.50.6540.6990.6770.517 1.250.4230.4110.4170.257 0.6250.2660.2550.2610.101 0.3130.2030.2110.2070.047 00.1550.1640.160 | |||||||
货期：3-5 working days
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