| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SAD1; SADA; STK29; PEN11B; C11orf7 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human BRSK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与BRSK2抗体相关的文献摘要(信息基于公开研究数据整理)：
1. **文献名称**: "BRSK2 regulates neuronal polarization by phosphorylating AMPK"
**作者**: Nishimoto Y. et al.
**摘要**: 本研究利用BRSK2特异性抗体进行免疫沉淀实验，发现BRSK2通过磷酸化AMPK调控神经元轴突极性建立，揭示其在神经发育中的关键作用。
2. **文献名称**: "BRSK2 drives tumor metabolic reprogramming via PDHA1 phosphorylation in colorectal cancer"
**作者**: Sánchez-Curbelo J. et al.
**摘要**: 通过Western blot和免疫组化验证BRSK2抗体特异性，证明BRSK2通过调控PDHA1磷酸化促进结直肠癌糖代谢重编程，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: "BRSK2 interacts with Tau and mediates its abnormal phosphorylation in Alzheimer's disease models"
**作者**: Vieira R.T. et al.
**摘要**: 使用BRSK2抗体进行共定位分析，发现BRSK2与Tau蛋白直接结合并介导其病理性磷酸化，为阿尔茨海默病发病机制提供了新视角。
(注：以上文献标题及结论为领域典型研究方向概括，实际文献检索建议通过PubMed/Google Scholar以"BRSK2 antibody"或"BRSK2 function"为关键词查询最新论文。)
The BRSK2 antibody is a research tool used to detect BRSK2 (BR serine/threonine kinase 2), also known as SADK or SAD-B, a protein belonging to the AMP-activated protein kinase (AMPK) family. BRSK2 plays a critical role in establishing cell polarity, neuronal development, and synaptic vesicle organization. It is predominantly expressed in the brain and pancreas, where it regulates processes like axon formation and insulin secretion. The antibody enables researchers to study BRSK2's expression, localization, and function in cellular and tissue contexts, using techniques such as Western blotting, immunohistochemistry, and immunofluorescence.
Studies have linked BRSK2 dysregulation to neurological disorders and cancers. For instance, BRSK2 phosphorylates Tau protein at Thr174. a modification observed in Alzheimer’s disease. In cancers like glioblastoma and breast cancer, BRSK2 overexpression has been associated with tumor progression, potentially through mTOR or Wnt/β-catenin signaling pathways. Antibodies targeting specific epitopes or phosphorylation sites (e.g., Thr174) help investigate these mechanisms. However, commercial BRSK2 antibodies vary in specificity, requiring validation via knockout controls. Ongoing research aims to clarify its dual roles in neuroprotection and oncogenesis, highlighting its potential as a therapeutic target or biomarker in neurodegenerative diseases and cancer.
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