| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BCA3; C11orf17 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human AKIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AKIP1抗体的3篇参考文献示例(文献信息为模拟内容，供参考)：
1. **"AKIP1 promotes breast cancer metastasis by modulating mitochondrial bioenergetics"**
- **作者**: Zhang Y, et al.
- **摘要**: 研究通过AKIP1抗体进行Western blot和免疫荧光实验，发现AKIP1通过增强线粒体能量代谢促进乳腺癌细胞侵袭和转移，并揭示其与HIF-1α信号通路的相互作用。
2. **"AKIP1 is a novel prognostic marker in colorectal cancer via regulating NF-κB pathway"**
- **作者**: Liu X, et al.
- **摘要**: 利用AKIP1抗体进行免疫组化分析结直肠癌组织样本，发现AKIP1高表达与患者预后不良相关，并通过调控NF-κB通路促进肿瘤炎症微环境形成。
3. **"AKIP1 modulates cardiac myocyte apoptosis via interaction with GRK2 in ischemia-reperfusion injury"**
- **作者**: Wang H, et al.
- **摘要**: 采用AKIP1抗体检测心肌细胞中AKIP1的定位及表达水平，揭示其在心脏缺血再灌注损伤中通过GRK2依赖性机制抑制细胞凋亡的保护作用。
4. **"AKIP1 antibody-based screening identifies its role in alternative splicing regulation"**
- **作者**: Chen R, et al.
- **摘要**: 通过AKIP1抗体进行免疫沉淀结合RNA测序，发现AKIP1与剪接因子相互作用，调控癌症相关基因(如TP53)的可变剪接，影响肿瘤细胞耐药性。
(注：以上文献为示例性内容，实际引用需查询真实数据库如PubMed、Web of Science等。)
AKIP1 (A Kinase Interacting Protein 1) is a regulatory protein that interacts with protein kinase A (PKA), a key enzyme in cellular signaling pathways. It modulates PKA activity by influencing its subcellular localization and substrate specificity, particularly under stress conditions. AKIP1 is involved in critical processes such as cell survival, proliferation, and apoptosis, and has been implicated in pathways like NF-κB signaling. Its expression is notably upregulated in certain cancers, including breast and colorectal cancer, where it correlates with poor prognosis and therapeutic resistance.
AKIP1 antibodies are essential tools for studying its expression, localization, and functional roles. These antibodies enable detection of AKIP1 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. High-quality AKIP1 antibodies are validated for specificity, often using knockout cell lines to confirm minimal cross-reactivity. Researchers employ these antibodies to explore AKIP1's involvement in disease mechanisms, such as its role in promoting cancer metastasis or enhancing cardiomyocyte survival during ischemic stress.
The study of AKIP1 and its antibodies holds therapeutic potential, particularly in targeting cancer progression and improving cardiac outcomes. Ongoing research aims to elucidate its molecular interactions and regulatory networks, which may lead to novel biomarkers or therapeutic strategies in oncology and cardiovascular diseases.
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