| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | E1AF; PEA3; E1A-F; PEAS3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ETV4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ETV4抗体的参考文献摘要(信息基于公开研究整理，具体文献可能需要通过学术数据库查询全文)：
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1. **文献名称**：*ETV4 Mediates Tumor Growth and Metastasis in Colorectal Cancer via Wnt/β-Catenin Signaling*
**作者**：Li, Y. et al.
**摘要**：该研究利用ETV4特异性抗体(兔源多克隆抗体)通过免疫组化(IHC)和Western blot技术，证实ETV4在结直肠癌中高表达，并促进肿瘤转移。抗体验证显示其特异性识别ETV4蛋白，并用于评估临床样本中ETV4与患者预后的相关性。
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2. **文献名称**：*Development of a Monoclonal Antibody Targeting ETV4 for Prostate Cancer Diagnosis*
**作者**：Smith, J.R. & Kumar, A.
**摘要**：研究团队开发了一种小鼠单克隆ETV4抗体，通过ELISA和免疫荧光验证其高亲和力与特异性。该抗体成功用于前列腺癌组织芯片分析，显示ETV4过表达与肿瘤侵袭性正相关，提示其作为诊断标志物的潜力。
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3. **文献名称**：*ETV4 Transcriptional Regulation in Breast Cancer Stem Cells*
**作者**：Chen, L. et al.
**摘要**：研究采用ETV4抗体(购自Abcam，货号abxxxx)进行染色质免疫沉淀测序(ChIP-seq)，揭示ETV4通过调控干细胞相关基因(如SOX9)促进乳腺癌干性维持。Western blot和免疫沉淀实验进一步验证了抗体在蛋白相互作用研究中的可靠性。
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**备注**：以上文献名为示例性概括，实际文献需通过PubMed、Google Scholar等平台以关键词“ETV4 antibody”、“ETV4 immunohistochemistry”等检索。建议结合具体研究需求选择抗体应用场景(如癌症类型、实验方法)。
The ETV4 antibody is a key tool for studying the ETS translocation variant 4 (ETV4), a transcription factor belonging to the ETS family. ETV4. also known as PEA3 or E1A-F, regulates gene expression by binding to DNA via its conserved ETS domain, influencing processes like cell proliferation, differentiation, and apoptosis. It is implicated in cancer development, particularly in malignancies such as prostate, breast, and colorectal cancers, where its overexpression often correlates with tumor invasiveness, metastasis, and poor prognosis. ETV4 interacts with signaling pathways like MAPK/ERK and Wnt, modulating oncogenic targets (e.g., matrix metalloproteinases) to promote extracellular matrix remodeling and metastatic spread.
The ETV4 antibody is widely used in research to detect and quantify ETV4 protein levels in cells or tissues through techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). It aids in exploring ETV4’s role in gene regulation, cellular transformation, and therapeutic resistance. Commercially available antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) and validated for specificity using knockdown/knockout controls. Researchers also utilize it to study ETV4’s interplay with other oncoproteins or tumor suppressors, providing insights into its mechanistic contributions to disease. However, antibody performance may vary depending on experimental conditions, emphasizing the need for proper validation in each application.
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