Human Dickkopf 3,DKK3 ELISA Kit

中文名： 人Dickkopf 3(DKK3)ELISA Kit

货号: IPD-E13607h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人Dickkopf 3(DKK3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DKK3含量。DKK3是一种分泌蛋白，属于Dickkopf家族，具有抑制Wnt/β-catenin信号通路的功能。其在发育、癌症、心血管疾病和组织再生中发挥重要作用。研究机制涉及抑制肿瘤细胞生长、促进血管修复和再生等。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。该产品适用于科研场景中DKK3相关机制研究，如探究其在肿瘤微环境、纤维化病理进程中的动态变化，或用于药物开发中靶向治疗效果评估。为疾病模型构建、生物标志物筛选等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Dickkopf 3 ELISA Kit; Dickkopf 3 homolog (Xenopus laevis) ELISA Kit; Dickkopf 3 homolog ELISA Kit; Dickkopf homolog 3 ELISA Kit; Dickkopf related protein 3 ELISA Kit; Dickkopf related protein 3 Precursor ELISA Kit; Dickkopf WNT signaling pathway inhibitor 3 ELISA Kit; Dickkopf; Xenopus; homolog of; 3 ELISA Kit; Dickkopf-3 ELISA Kit; Dickkopf-related protein 3 ELISA Kit; Dickkopf3 ELISA Kit; DKK 3 ELISA Kit; Dkk-3 ELISA Kit; DKK3 ELISA Kit; DKK3_HUMAN ELISA Kit; hDkk 3 ELISA Kit; hDkk-3 ELISA Kit; hDkk3 ELISA Kit; Regulated in glioma ELISA Kit; REIC ELISA Kit; RIG ELISA Kit; RIG-like 5-6 ELISA Kit; RIG-like 7-1 ELISA Kit
缩写：
DKK3
Uniprot No.：
Q9UBP4
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.2 pg/mL-2000 pg/mL
灵敏度：
7.8 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Stem Cells
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)251.396250.357253.964250.306248.944253.061SD0.0400.0400.0500.0580.0630.064CV(%)4.9084.7905.9316.7687.3777.610

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DKK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:50

Average %

Range %

86-112

1:100

Average %

Range %

88-98

1:200

Average %

Range %

90-113

1:400

Average %

100

Range %

93-115

回收率：

The recovery of human DKK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	89-106
EDTA plasma (n=4)	93	86-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
2000	2.811	2.804	2.807	2.669
1000	2.041	2.014	2.028	1.890
500	1.268	1.258	1.263	1.125
250	0.808	0.842	0.825	0.687
125	0.592	0.587	0.589	0.451
62.5	0.321	0.334	0.327	0.189
31.2	0.267	0.261	0.264	0.126
0	0.155	0.151	0.153