Human Delta-like protein 1(DLL1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL006947HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人δ样蛋白1(DLL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的DLL1含量。DLL1（Delta-like protein 1）是一种单次跨膜糖蛋白，属于DSL蛋白家族，作为Notch信号转导通路的配体之一，与Notch受体结合激活Notch信号通路，决定细胞分化，调控多种组织生长发育。DLL1在肿瘤生长、分化、增殖及凋亡中发挥重要作用，与肿瘤耐药密切相关。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.8 pg/ml。适用于细胞生物学、分子病理学等领域的科研需求，例如探究肿瘤发生机制、评估组织特异性蛋白表达模式或验证基因编辑模型中的分子调控效果。为研究DLL1在疾病模型或信号转导网络中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
DLL1; UNQ146/PRO172; Delta-like protein 1; Drosophila Delta homolog 1; Delta1; H-Delta-1
缩写：
DLL1
Uniprot No.：
O00548
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
31.25 pg/ml - 2000 pg/ml
灵敏度：
7.8 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Developmental Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DLL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	85
	Range %	81-89
1:4	Average %	110
	Range %	107-113
1:8	Average %	96
	Range %	93-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.400	2.468	2.434	2.292
1000	1.795	1.917	1.856	1.714
500	1.268	1.259	1.264	1.122
250	0.732	0.708	0.720	0.578
125	0.452	0.468	0.460	0.318
62.5	0.288	0.296	0.292	0.150
31.25	0.202	0.210	0.206	0.064
0	0.140	0.143	0.142