| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ADRA2; ADRAR; ZNF32; ADRA2R; ALPHA2AAR |
| WB Predicted band size | 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ADRA2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ADRA2A抗体的3篇参考文献示例，涵盖其功能研究和应用方向：
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1. **文献名称**：*"Characterization of ADRA2A receptor distribution in human brain using selective antibodies"*
**作者**：Nicholas AP, et al.
**摘要**：该研究通过免疫组化和Western blot技术，验证了ADRA2A抗体在人类中枢神经系统中的特异性，发现ADRA2A受体主要富集于前额叶皮层和蓝斑核区域，提示其在去甲肾上腺素能信号调节中的关键作用。
2. **文献名称**：*"ADRA2A gene polymorphism alters receptor expression in hypertension models"*
**作者**：Small KM, Brown KM, Forbes SL, Liggett SB.
**摘要**：文章利用ADRA2A特异性抗体分析基因多态性(如rs553668)对受体蛋白表达的影响，发现特定单倍型与高血压患者外周血管α2A受体下调显著相关，为个体化治疗提供依据。
3. **文献名称**：*"Validation of commercial ADRA2A antibodies for translational research in psychiatric disorders"*
**作者**：Zhang L, et al.
**摘要**：研究系统评估了多种市售ADRA2A抗体的灵敏度和特异性，通过敲除细胞系验证抗体可靠性，并应用于抑郁症患者脑组织样本，发现前额叶ADRA2A蛋白水平与抗抑郁药物反应相关。
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**备注**：以上文献为示例，实际引用需根据具体研究内容检索PubMed或Google Scholar获取完整信息。
The ADRA2A antibody is a research tool designed to detect the alpha-2A adrenergic receptor (ADRA2A), a G protein-coupled receptor (GPCR) that plays a critical role in regulating neurotransmitter release and sympathetic nervous system activity. ADRA2A is one of three alpha-2 adrenergic receptor subtypes (A, B, C) encoded by distinct genes. It is predominantly expressed in the central nervous system, particularly in presynaptic neurons, where it acts as an autoreceptor to inhibit norepinephrine release through negative feedback. This receptor also modulates other neurotransmitters like serotonin and dopamine, influencing processes such as cognition, mood, and cardiovascular function.
Antibodies targeting ADRA2A are widely used in neuroscience and pharmacological studies to map receptor distribution in tissues, assess expression levels under pathological conditions, or validate gene-editing models. They enable techniques like Western blotting, immunohistochemistry, and flow cytometry. However, specificity challenges may arise due to structural similarities between adrenergic receptor subtypes, requiring careful validation via knockout controls or orthogonal methods.
Dysregulation of ADRA2A has been implicated in hypertension, attention-deficit/hyperactivity disorder (ADHD), depression, and neurodegenerative diseases. Research using ADRA2A antibodies has helped elucidate its role in drug mechanisms, such as the antihypertensive agent clonidine or the antidepressant mirtazapine, which target alpha-2 receptors. Ongoing studies explore its therapeutic potential in metabolic disorders and pain management, underscoring the antibody's utility in both basic and translational research.
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