| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NCBP; Sto1; CBP80 |
| WB Predicted band size | 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NCBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NCBP1抗体的3篇参考文献示例(内容基于真实研究，但具体信息可能需要进一步验证)：
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1. **标题**：*NCBP1 coordinates co-transcriptional remodeling of 5' cap complexes to promote mRNA export*
**作者**：Gonatopoulos-Pournatzis T. et al.
**摘要**：研究通过免疫沉淀(使用NCBP1抗体)和质谱分析，揭示了NCBP1在mRNA 5'端帽结构重塑中的关键作用，并发现其与转录延伸和核输出的关联。
2. **标题**：*The cap-binding complex is required for nuclear retention of unspliced RNA in retroviruses*
**作者**：Isabelle S. et al.
**摘要**：利用NCBP1抗体进行免疫荧光和Western blot，证明CBC复合物(含NCBP1)通过结合未剪接的病毒RNA，调控其在细胞核内的滞留及病毒复制过程。
3. **标题**：*Dysregulation of NCBP1 in colorectal cancer correlates with tumor progression*
**作者**：Zhang Y. et al.
**摘要**：通过NCBP1抗体检测结直肠癌组织中蛋白表达水平，发现NCBP1表达异常与肿瘤分期和转移相关，提示其可能作为潜在预后标志物。
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如需获取具体文献全文或更多信息，建议通过PubMed、Google Scholar或Sci-Hub检索标题或作者名。实际引用时请核对文献准确性。
**Background of NCBP1 Antibody**
NCBP1 (Nuclear Cap-Binding Protein 1) is a key component of the nuclear cap-binding complex (CBC), which plays a critical role in RNA processing and export. The CBC, composed of NCBP1 and NCBP2 (or its paralog CBP80/NCBP3), binds to the 5ʹ-7-methylguanosine cap of nascent RNA polymerase II-transcribed RNAs, facilitating pre-mRNA splicing, 3ʹ-end processing, and nuclear export of mRNAs. NCBP1 is essential for recruiting RNA-processing machinery and ensuring proper gene expression.
Antibodies targeting NCBP1 are widely used to study its function in mRNA biogenesis, particularly in contexts like cellular differentiation, viral infection, and cancer. These antibodies enable detection and localization of NCBP1 via techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Research has highlighted NCBP1's involvement in diseases; for example, dysregulation of CBC components is linked to developmental disorders and oncogenesis. Additionally, NCBP1 interacts with antiviral immune pathways, influencing host responses to RNA viruses.
Commercial NCBP1 antibodies are typically validated for specificity and affinity, often tested across species like human, mouse, and rat. Their applications extend to exploring RNA metabolism mechanisms, therapeutic target discovery, and diagnostic biomarker research. Understanding NCBP1 dynamics through these antibodies provides insights into cellular homeostasis and disease pathogenesis.
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