| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SPG28; PAPLA1; PA-PLA1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DDHD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是假设性的参考文献示例，供参考(实际文献需通过学术数据库验证)：
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1. **"DDHD1 regulates lipid droplet dynamics and mitochondrial function in murine models"**
*Shirane et al. (2015), Journal of Lipid Research*
摘要：研究利用DDHD1特异性抗体，通过Western blot和免疫组化分析DDHD1在小脑和肝脏中的表达，发现其缺失导致脂滴积累及线粒体功能障碍。
2. **"Characterization of a polyclonal antibody against human DDHD1 for functional studies in neurodegenerative disorders"**
*Lee et al. (2018), Experimental Cell Research*
摘要：开发并验证一种兔源多克隆抗体，用于免疫沉淀和免疫荧光，证实DDHD1在神经元中的亚细胞定位及其与脂代谢异常疾病的关联。
3. **"Comparative analysis of DDHD1 antibody specificity across mammalian cell lines"**
*Smith et al. (2019), Molecular Biology Reports*
摘要：比较三种商用DDHD1抗体的性能，发现抗体X在HeLa和HEK293细胞中特异性最佳，为后续功能研究提供工具验证。
4. **"DDHD1 mutations impair phosphatidic acid metabolism in hereditary spastic paraplegia"**
*Garcia et al. (2020), Human Molecular Genetics*
摘要：使用DDHD1抗体检测患者成纤维细胞蛋白水平，揭示突变导致酶活性丧失及磷脂酸代谢紊乱，为疾病机制提供依据。
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**注**：以上为模拟示例，实际文献需查阅PubMed、Google Scholar等平台。建议用关键词“DDHD1 antibody”、“DDHD1 function”或“DDHD1 knockout”检索最新研究。
**Background of DDHD1 Antibody**
DDHD1 (Dual Domain HD-Containing Protein 1) is a phospholipase enzyme encoded by the *DDHD1* gene, belonging to the intracellular phospholipase A1 (PLA1) family. It plays a critical role in lipid metabolism, particularly in hydrolyzing phospholipids to generate bioactive lipid mediators. DDHD1 is implicated in membrane trafficking, organelle dynamics, and lipid droplet regulation, with prominent expression in the brain, testes, and liver.
Research links DDHD1 dysfunction to neurological disorders, including hereditary spastic paraplegia (HSP), a condition characterized by motor neuron degeneration. Mutations in *DDHD1* are associated with HSP subtype SPG28. highlighting its role in maintaining neuronal integrity. Additionally, DDHD1's involvement in lipid signaling pathways suggests potential connections to metabolic syndromes and cancer progression.
DDHD1 antibodies are essential tools for studying the protein's expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in investigations of its physiological and pathological roles. Commercial DDHD1 antibodies are typically developed against specific epitopes, often validated for specificity in knockout controls. Reliable antibodies help unravel DDHD1's interactions with lipid substrates, its regulatory mechanisms, and its contribution to disease pathways, offering insights for therapeutic targeting in lipid-related disorders.
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