| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RFG5; GOLIM5; ret-II |
| WB Predicted band size | 83 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GOLGA5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及GOLGA5抗体的文献示例(部分信息为模拟简化版，实际引用需核实原文)：
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1. **文献名称**: *GOLGA5 as a novel biomarker in prostate cancer: Golgi structure and antibody validation*
**作者**: Smith J, et al.
**摘要**: 本研究通过开发兔源多克隆GOLGA5抗体，验证其在前列腺癌细胞中定位高尔基体的特异性。Western blot检测到约75 kDa的条带，免疫荧光显示癌细胞中高尔基体碎片化，提示GOLGA5表达异常与癌症进展相关。
2. **文献名称**: *Characterization of GOLGA5 autoantibodies in autoimmune disorders*
**作者**: Lee S, et al.
**摘要**: 利用重组人GOLGA5蛋白筛选系统性红斑狼疮患者血清，发现部分患者存在抗GOLGA5自身抗体。研究提示此类抗体可能干扰高尔基体功能，为自身免疫病理机制提供新线索。
3. **文献名称**: *Role of GOLGA5 in neuronal development: Insights from antibody-mediated knockdown*
**作者**: Chen R, et al.
**摘要**: 采用单克隆GOLGA5抗体阻断蛋白功能，发现其抑制神经元突触形成，证明GOLGA5通过调控囊泡运输参与神经发育，抗体工具为研究高尔基体动态提供关键手段。
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**注意**：以上为示例模板，实际文献需通过PubMed/Google Scholar检索关键词“GOLGA5 antibody”或“GOLGA5 immunolocalization”获取，并核对作者、期刊及具体实验细节。
The Golgin A5 (GOLGA5) antibody is a tool used to detect GOLGA5. a member of the golgin family of proteins localized to the Golgi apparatus. GOLGA5. also known as RET ligand 4 (RETL4), is characterized by its coiled-coil domains and plays a role in maintaining Golgi structure, vesicle trafficking, and protein sorting. It interacts with other golgins and Rab GTPases to mediate tethering and fusion events critical for Golgi organization.
GOLGA5 antibodies are widely utilized in research to study Golgi dynamics, cellular stress responses, and cancer biology. Notably, GOLGA5 gained attention due to its involvement in chromosomal rearrangements, particularly the RET-GOLGA5 fusion in thyroid and other cancers, which drives oncogenic signaling. Antibodies targeting GOLGA5 help identify such fusion proteins and assess Golgi integrity in disease models.
In autoimmune contexts, anti-GOLGA5 autoantibodies have been reported in subsets of patients with Sjögren’s syndrome or systemic lupus erythematosus, though their clinical significance remains under investigation. Commercial GOLGA5 antibodies are validated for techniques like Western blotting, immunofluorescence, and immunohistochemistry, often serving as Golgi markers. Researchers must consider potential cross-reactivity with homologous golgins and validate specificity using knockout controls. Ongoing studies explore its roles in neurodegeneration, viral infection, and Golgi-targeted therapies.
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