| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FFA2R; GPR43 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human FFAR2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FFAR2抗体的示例参考文献(内容为假设性示例，仅供参考)：
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1. **文献名称**：*FFAR2 Antibody-Based Detection of Short-Chain Fatty Acid Receptor Expression in Colonic Epithelium*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种高特异性兔源多克隆FFAR2抗体，通过免疫组化和Western blot验证其在人结肠上皮细胞中的表达。结果显示FFAR2在肠道屏障功能中可能通过响应短链脂肪酸调节炎症反应。
2. **文献名称**：*A Monoclonal Antibody Targeting FFAR2 Attenuates Obesity-Related Metabolic Inflammation*
**作者**：Zhang Y, et al.
**摘要**：作者制备了一种小鼠抗人FFAR2单克隆抗体，并证明其能有效阻断受体活性。在肥胖模型中，该抗体减少了脂肪组织巨噬细胞浸润，提示FFAR2在代谢炎症中的潜在治疗价值。
3. **文献名称**：*Characterization of FFAR2-Specific Antibodies for Flow Cytometry Applications*
**作者**：Brown JE, et al.
**摘要**：本文系统比较了多种商用FFAR2抗体的特异性，发现克隆7G8在流式细胞术中表现最佳。研究为免疫细胞表面FFAR2的精准检测提供了方法学参考。
4. **文献名称**：*FFAR2 Neutralizing Antibody Suppresses NLRP3 Inflammasome Activation in Macrophages*
**作者**：Wang L, et al.
**摘要**：通过中和抗体抑制FFAR2信号，证实了短链脂肪酸-FFAR2轴调控巨噬细胞NLRP3炎症小体活化的机制，为靶向FFAR2的免疫调节策略提供依据。
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注：以上文献为示例，实际引用时请以真实发表的论文为准。建议通过PubMed、Web of Science等数据库检索关键词“FFAR2 antibody”或“GPR43 antibody”获取具体文献。
FFAR2 (Free Fatty Acid Receptor 2), also known as GPR43. is a G protein-coupled receptor that binds short-chain fatty acids (SCFAs) like acetate, propionate, and butyrate, which are produced by gut microbiota through dietary fiber fermentation. It plays a critical role in immune regulation, metabolic homeostasis, and gut-brain axis communication. FFAR2 is highly expressed in immune cells (e.g., neutrophils, macrophages), intestinal epithelial cells, and adipose tissue, where it modulates inflammatory responses, insulin secretion, and energy metabolism.
Antibodies targeting FFAR2 are essential tools for studying its expression, localization, and signaling mechanisms. They enable researchers to investigate its dual coupling to Gαi/o (inhibiting cAMP) and Gαq (activating intracellular calcium), as well as its role in diseases like inflammatory bowel disease (IBD), obesity, and diabetes. FFAR2 agonists or antagonists are explored for therapeutic potential, but selective antibodies help dissect receptor-specific effects in complex pathways. Challenges include ensuring specificity due to homology with FFAR3 (GPR41). Current research leverages FFAR2 antibodies in immunoassays, flow cytometry, and immunohistochemistry to unravel its tissue-specific functions and microbiota-host interactions, highlighting its relevance in precision medicine and metabolic disorder therapeutics.
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