| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GPR78 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GPR78抗体的3篇模拟参考文献示例(实际文献可能需要通过数据库验证)：
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1. **文献名称**：*"Characterization of GPR78 Antibody Specificity in Stress Response Pathways"*
**作者**：Lin, Y. et al.
**摘要**：本研究通过Western blot和免疫荧光验证了GPR78抗体的特异性，发现GPR78在内质网应激条件下表达上调，与未折叠蛋白反应(UPR)通路中的PERK信号相关。抗体成功检测到HEK293细胞和小鼠肝脏组织中的GPR78蛋白。
2. **文献名称**：*"GPR78 as a Novel Biomarker in Glioblastoma: Validation via Immunohistochemistry"*
**作者**：Smith, J.R. & Wang, L.
**摘要**：利用商业GPR78抗体对胶质母细胞瘤组织样本进行免疫组化分析，发现GPR78高表达与患者不良预后相关。研究证实该抗体在区分肿瘤与正常脑组织中的特异性，并提示GPR78可能通过调控MAPK通路促进肿瘤侵袭。
3. **文献名称**：*"Role of GPR78 in Neuronal Apoptosis: Insights from Antibody-Based Knockdown Studies"*
**作者**：Kokame, K. et al.
**摘要**：通过抗体制备的siRNA复合物靶向敲低GPR78.发现其缺失加剧了氧化应激诱导的神经元凋亡。抗体验证显示GPR78在皮层神经元中广泛表达，且与Bcl-2家族蛋白存在相互作用，提示其可能参与神经保护机制。
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**说明**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索(关键词：GPR78 antibody, GPR78 function)。建议结合具体研究目的筛选抗体应用(如WB、IHC)或功能研究相关的论文。
GPR78 (G protein-coupled receptor 78) is a member of the adhesion G protein-coupled receptor (GPCR) subfamily, characterized by large extracellular regions involved in cell-cell or cell-matrix interactions. As an orphan receptor, its endogenous ligands and precise physiological roles remain poorly understood. Studies suggest GPR78 is broadly expressed in tissues, including the brain, cardiovascular system, and endocrine organs, implicating potential roles in neuroregulation, metabolic processes, and stress responses. It has been linked to pathways modulating cAMP signaling, cellular adhesion, and possibly tumor progression, though mechanistic insights are limited.
GPR78 antibodies are critical tools for investigating its expression patterns, subcellular localization, and interactions. Commonly used in techniques like Western blotting, immunohistochemistry, and flow cytometry, these antibodies help elucidate GPR78's tissue distribution and dynamic regulation under pathological conditions, such as cancer, metabolic disorders, or neurological diseases. Commercial GPR78 antibodies are typically raised against specific epitopes, often in the extracellular or intracellular domains, with validation in knockdown models to confirm specificity. Challenges include cross-reactivity due to homology with other GPCRs and limited functional data correlating antibody-based findings with receptor activity. Ongoing research aims to clarify GPR78's signaling mechanisms and therapeutic potential, positioning it as a target for drug discovery in conditions like obesity, neurodegeneration, or inflammation.
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