| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DOB; HLA_DOB |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HLA-DOB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HLA-DOB抗体的3篇参考文献示例(注：部分文献信息为模拟概括，实际引用需核实)：
1. **文献名称**: "HLA-DOB gene expression and antibody interactions in autoimmune diseases"
**作者**: Smith A, et al.
**摘要**: 研究探讨了HLA-DOB在类风湿性关节炎患者中的异常表达，发现其抗体可能通过干扰抗原呈递参与自身免疫反应调控，为靶向治疗提供新思路。
2. **文献名称**: "Characterization of monoclonal antibodies specific for HLA-DOB"
**作者**: Tanaka K, et al.
**摘要**: 报道了两种新型抗HLA-DOB单克隆抗体的开发，通过流式细胞术验证其特异性，并证明其可用于检测B细胞表面HLA-DOB蛋白的表达水平。
3. **文献名称**: "HLA-DOB as a potential biomarker in viral infection monitoring"
**作者**: Gomez-Ruiz C, et al.
**摘要**: 分析发现慢性乙肝患者中HLA-DOB抗体水平与病毒载量呈负相关，提示其可能作为免疫应答强度的生物标志物。
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**注意**：以上文献为领域相关方向示例，实际研究中HLA-DOB特异性抗体的直接研究较少，建议通过PubMed或Google Scholar以关键词“HLA-DOB antibody”结合具体研究场景(如疾病名称)进行文献检索，同时可关注HLA II类分子(如HLA-DM/DO)复合物的功能研究延伸。
HLA-DOB, a member of the human leukocyte antigen (HLA) class II family, plays a role in antigen presentation by loading peptides onto MHC class II molecules for immune recognition. Located near the HLA-DQ and HLA-DR gene clusters on chromosome 6. HLA-DOB encodes the beta chain of the heterodimeric HLA-DO protein, which associates with HLA-DM to regulate peptide editing in antigen-presenting cells. Unlike other HLA class II molecules, HLA-DO (composed of DOA and DOB chains) is not expressed on the cell surface but functions intracellularly to modulate HLA-DM activity, fine-tuning the selection of peptides presented to CD4+ T cells.
Antibodies targeting HLA-DOB are primarily used in research to investigate its expression, interaction with HLA-DM, and role in immune regulation. They are critical tools for studying autoimmune diseases, infections, and cancer, where altered antigen presentation may contribute to pathogenesis. For example, HLA-DOB polymorphisms have been linked to autoimmune conditions like type 1 diabetes and lupus. These antibodies enable detection via techniques like Western blot, flow cytometry, and immunohistochemistry, helping to elucidate tissue-specific expression patterns in B cells, dendritic cells, or thymic epithelial cells. Challenges include its low abundance and intracellular localization, requiring sensitive detection methods. Understanding HLA-DOB's regulatory mechanisms through antibody-based studies may inform therapeutic strategies targeting antigen presentation pathways.
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