| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HRG22; ZBTB30; ZNF907 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HIC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HIC2抗体的3篇参考文献(示例为虚构内容，实际文献需根据数据库查询)：
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1. **文献名称**: "HIC2 regulates cardiac development through transcriptional activation of myocyte enhancer factors"
**作者**: Smith A, et al. (2021)
**摘要**: 本研究利用HIC2特异性抗体进行免疫共沉淀和染色质免疫荧光分析，发现HIC2通过结合心脏发育相关基因的启动子区域调控其表达，在小鼠模型中证实其缺失导致心脏形态异常。
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2. **文献名称**: "Epigenetic silencing of HIC2 by hypermethylation promotes tumor progression in colorectal cancer"
**作者**: Johnson R, et al. (2020)
**摘要**: 通过Western blot和免疫组化(使用HIC2单克隆抗体)发现，结直肠癌组织中HIC2蛋白表达显著下调，其启动子高甲基化状态与患者不良预后相关，体外实验表明HIC2抑制肿瘤细胞增殖。
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3. **文献名称**: "HIC2 interacts with BCL6 to modulate chromatin remodeling in B-cell lymphomas"
**作者**: Lee S, et al. (2019)
**摘要**: 研究采用HIC2抗体进行ChIP-seq和免疫共沉淀实验，揭示HIC2与BCL6形成复合物，通过调节染色质结构影响淋巴瘤细胞的基因表达谱，为靶向治疗提供新思路。
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4. **文献名称**: "Dynamic subcellular localization of HIC2 during the cell cycle revealed by antibody-based imaging"
**作者**: Brown K, et al. (2018)
**摘要**: 利用HIC2多克隆抗体进行免疫荧光和流式细胞术分析，发现HIC2在细胞周期不同阶段的核内定位存在动态变化，提示其可能参与DNA复制或损伤修复过程。
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(注：以上文献为示例，实际引用需查询PubMed、Google Scholar等数据库获取真实信息。)
The HIC2 (Hypermethylated in Cancer 2) antibody is a tool used to study the HIC2 protein, encoded by the HIC2 gene located on human chromosome 22q11.21. HIC2 functions as a transcriptional regulator belonging to the BTB/POZ zinc finger protein family, implicated in diverse biological processes, including cell proliferation, differentiation, and apoptosis. Initially identified as a tumor suppressor, HIC2 is frequently epigenetically silenced via hypermethylation in various cancers, such as colorectal and breast cancer, correlating with poor prognosis. Beyond oncology, HIC2 plays critical roles in cardiovascular development, particularly in heart morphogenesis and outflow tract septation, as evidenced by studies in mouse models.
The HIC2 antibody is widely utilized in research to detect and quantify HIC2 protein expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It aids in exploring HIC2's interaction networks, subcellular localization (primarily nuclear), and regulatory mechanisms. Researchers also employ this antibody to investigate HIC2's role in DNA damage response and its cross-talk with signaling pathways like p53 and Wnt. Commercial HIC2 antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown. Despite its utility, challenges remain in standardizing detection protocols across tissue types due to variable expression levels. Ongoing studies aim to clarify HIC2's dual roles in tumor suppression and developmental biology, highlighting its potential as a therapeutic target or diagnostic marker.
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