| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PAIP-2; PAIP2A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PAIP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于PAIP2抗体的参考文献示例(内容基于模拟学术文献，可能需根据实际研究调整)：
1. **文献名称**：*PAIP2 interacts with poly(A)-binding protein through two independent binding motifs*
**作者**：Roy G, De Crescenzo G, Khaleghpour K et al.
**摘要**：本研究通过免疫共沉淀和Western blot技术，利用PAIP2特异性抗体验证了PAIP2与PABP(poly(A)-binding protein)的相互作用，揭示了二者在翻译调控中的功能关联。
2. **文献名称**：*Regulation of maternal mRNA translation by PAIP2 during oocyte maturation*
**作者**：Martineau Y, Derry MC, Wang X et al.
**摘要**：通过PAIP2抗体进行免疫荧光和蛋白质表达分析，发现PAIP2通过抑制PABP活性调控卵母细胞成熟过程中母源mRNA的翻译，影响早期胚胎发育。
3. **文献名称**：*PAIP2 suppresses tumor progression by destabilizing oncogenic mRNAs in colorectal cancer*
**作者**：Yao Y, Li X, Liu S et al.
**摘要**：采用PAIP2抗体进行免疫组化和Western blot，证明PAIP2在结直肠癌中低表达，其缺失通过稳定促癌mRNA促进肿瘤侵袭，提示其作为潜在生物标志物。
4. **文献名称**：*Subcellular localization and dynamic shuttling of PAIP2 in stress granules*
**作者**：Körner CG, Wormington M, Mühlemann O et al.
**摘要**：利用PAIP2特异性抗体结合共聚焦显微镜，发现PAIP2在细胞应激条件下易位至应激颗粒，调控mRNA稳定性及翻译重编程，揭示其在应激反应中的新机制。
注：以上文献为示例性质，实际引用需核对真实出版物信息及原文内容。
PAIP2 (Poly(A)-binding protein-interacting protein 2) is a regulatory protein involved in post-transcriptional gene expression, primarily through its interaction with poly(A)-binding proteins (PABPs). It modulates mRNA stability and translation by competitively binding to PABP, thereby disrupting the PABP-eIF4G complex essential for translation initiation. PAIP2 exists as two isoforms, PAIP2A and PAIP2B, generated via alternative splicing, with PAIP2A being more widely studied. Its expression is tightly regulated, influencing cellular processes such as synaptic plasticity, cell proliferation, and stress responses. Dysregulation of PAIP2 has been implicated in neurological disorders, cancer, and viral infections.
PAIP2 antibodies are crucial tools for investigating its expression, localization, and functional roles. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study PAIP2 in various tissues and disease models. These antibodies help elucidate mechanisms of translational control and identify PAIP2-associated pathways in pathologies. Commercial PAIP2 antibodies are typically raised against specific epitopes of human or murine PAIP2. with validation emphasizing specificity through knockout controls. Research utilizing these antibodies has advanced understanding of mRNA metabolism and highlighted PAIP2 as a potential therapeutic target in diseases linked to translational dysregulation.
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