| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PKCL; PKC-L; PRKCL; nPKC-eta |
| WB Predicted band size | 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRKCH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRKCH抗体的3篇代表性文献，简要概括如下：
1. **"Protein kinase C η as a prognostic marker in hepatocellular carcinoma"**
- **作者**: Ito Y, et al.
- **摘要**: 研究通过免疫组化和Western blot分析，发现PRKCH在肝癌组织中显著过表达，并与患者预后不良相关，提示其可作为潜在生物标志物。
2. **"PKCη promotes glioblastoma progression by regulating cell proliferation and invasion"**
- **作者**: Zhang L, et al.
- **摘要**: 利用PRKCH抗体进行功能实验，证实PKCη通过激活ERK信号通路促进胶质母细胞瘤细胞增殖和侵袭，提示其作为治疗靶点的可能性。
3. **"Autoantibodies against PRKCH in systemic lupus erythematosus"**
- **作者**: Sato S, et al.
- **摘要**: 研究发现系统性红斑狼疮患者血清中PRKCH自身抗体水平升高，可能参与疾病发病机制，为自身免疫诊断提供新方向。
4. **"Role of PKCη in epidermal growth factor receptor signaling"**
- **作者**: Chida K, et al.
- **摘要**: 通过抗体阻断实验，揭示PKCη在EGFR下游信号中的调控作用，影响表皮细胞分化和肿瘤发生。
以上文献涵盖了PRKCH抗体在癌症、自身免疫疾病及分子机制研究中的应用。如需具体年份或期刊，可进一步补充。
The PRKCH antibody targets protein kinase C eta (PKCη), a member of the PKC serine/threonine kinase family involved in diverse cellular processes. PKCη, encoded by the PRKCH gene, belongs to the novel PKC subfamily (nPKC), characterized by calcium-independent activation but dependence on diacylglycerol (DAG) or phorbol esters. It contains a regulatory C1 domain for lipid binding and a catalytic kinase domain.
PKCη plays critical roles in cell proliferation, differentiation, apoptosis, and immune regulation. It is highly expressed in epithelial tissues, lungs, and skin, and is implicated in cancer progression, neurodegenerative disorders, and inflammatory responses. Studies link PRKCH polymorphisms to autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. In cancer, PKCη may act as both oncogene and tumor suppressor, depending on context, influencing pathways like NF-κB and MAPK.
PRKCH antibodies are essential tools for detecting PKCη expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They aid in studying PKCη's functional dynamics in signaling cascades and its therapeutic potential. Commercial antibodies are validated for specificity against unique epitopes in human/mouse samples, enabling research into PKCη's role in disease mechanisms and drug development.
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