| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HIP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HHIP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HHIP抗体的3篇参考文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**: *HHIP Expression in Non-Small Cell Lung Cancer: Prognostic Implications*
**作者**: Yoon JW, et al.
**摘要**: 该研究通过免疫组化(使用HHIP抗体)分析非小细胞肺癌组织中HHIP蛋白的表达水平，发现其低表达与患者预后不良相关，提示HHIP可能作为抑癌基因发挥作用。
2. **文献名称**: *Role of HHIP in Embryonic Limb Development*
**作者**: Li H, et al.
**摘要**: 利用HHIP抗体进行Western blot和免疫荧光实验，研究小鼠胚胎肢体发育中HHIP的时空表达模式，揭示其通过拮抗Hedgehog信号通路调控骨骼形成。
3. **文献名称**: *Epigenetic Regulation of HHIP in Pancreatic Cancer*
**作者**: Xu J, et al.
**摘要**: 通过HHIP抗体检测胰腺癌细胞系中蛋白表达，结合甲基化分析，发现HHIP启动子高甲基化导致其表达沉默，促进肿瘤侵袭转移。
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**注**：以上文献为示例，实际引用需核实具体来源及发表信息。建议通过PubMed或Google Scholar搜索关键词“HHIP antibody”或“Hedgehog interacting protein”获取最新研究。
The Hedgehog-interacting protein (HHIP) antibody is a crucial tool for studying the HHIP, a glycoprotein that acts as a key antagonist of the Hedgehog (Hh) signaling pathway. HHIP binds to all three Hh ligands (Sonic, Indian, and Desert Hedgehog), inhibiting their interaction with the Patched receptor and modulating pathway activity. This pathway is vital in embryonic development, tissue homeostasis, and stem cell regulation, but its dysregulation is implicated in cancers (e.g., medulloblastoma, basal cell carcinoma) and developmental disorders.
HHIP itself functions as a tumor suppressor, with reduced expression observed in lung, pancreatic, and liver cancers, often correlating with poor prognosis. Antibodies targeting HHIP enable researchers to detect its expression and localization via techniques like Western blot, immunohistochemistry, and immunofluorescence. These studies help elucidate HHIP's role in developmental biology, disease mechanisms, and therapeutic targeting. For instance, HHIP antibodies have been used to explore its involvement in chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis, where HHIP dysregulation contributes to pathological remodeling.
Research using HHIP antibodies also extends to regenerative medicine, particularly in understanding skeletal and neural development. By blocking Hh signaling, HHIP influences cell differentiation and tissue patterning, making it a focal point for studies on developmental anomalies. Overall, HHIP antibodies are indispensable for unraveling the dual role of HHIP as a modulator of critical signaling pathways and a potential biomarker or therapeutic target in diseases.
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