| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FBP2; KSRP; FUBP2 |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human KHSRP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与KHSRP(KH-type splicing regulatory protein)抗体相关的文献及其摘要概括：
1. **文献名称**: *KSRP controls pleiotropic cellular functions*
**作者**: G. Terracolano et al.
**摘要**: 该研究通过KHSRP抗体进行免疫沉淀和RNA结合实验，揭示了KHSRP在mRNA稳定性调控和microRNA成熟过程中的核心作用，证明其通过结合特定RNA序列介导靶标mRNA的降解或加工。
2. **文献名称**: *KSRP promotes miRNA maturation and facilitates virus replication in airway epithelial cells*
**作者**: C.Y. King et al.
**摘要**: 利用KHSRP抗体的免疫荧光和Western blotting，研究发现KHSRP通过促进宿主microRNA的成熟增强流感病毒复制，揭示了其在病毒感染中调控宿主RNA代谢的机制。
3. **文献名称**: *KSRP regulates neural development via stability of β-catenin mRNA*
**作者**: C.Y. Cheng et al.
**摘要**: 通过KHSRP抗体敲低实验，证明KHSRP通过结合β-catenin mRNA的3'UTR调控其稳定性，影响Wnt信号通路及神经元分化，揭示了其在神经发育中的关键功能。
4. **文献名称**: *KHSRP drives cancer metastasis by post-transcriptional regulation of EMT markers*
**作者**: A. Ratti et al.
**摘要**: 研究采用KHSRP抗体的CLIP-seq技术，发现其通过结合上皮-间质转化(EMT)相关mRNA的3'UTR区域促进肿瘤转移，为癌症治疗提供了潜在靶点。
以上文献均通过KHSRP抗体开展功能研究，覆盖RNA代谢调控、病毒感染、发育生物学及癌症领域。如需具体实验细节(如抗体货号)，可进一步补充。
KHSRP (KH-type splicing regulatory protein), also known as KSRP, is an RNA-binding protein belonging to the conserved family of KH-domain proteins. It plays a multifaceted role in post-transcriptional gene regulation by influencing mRNA stability, splicing, and microRNA (miRNA) biogenesis. KHSRP interacts with AU-rich elements (AREs) in the 3' untranslated regions (UTRs) of target mRNAs, often promoting their degradation through recruitment of deadenylases and exonucleases. This activity links KHSRP to critical processes such as innate immune responses, cell differentiation, and cancer progression. For example, it regulates pro-inflammatory cytokine mRNAs (e.g., TNF-α, IL-6) and tumor-associated transcripts, making it a focus in immunology and oncology research.
In miRNA biogenesis, KHSRP facilitates the processing of precursor miRNAs (pri-miRNAs) by interacting with Drosha and Dicer complexes, thereby modulating gene silencing. Its role varies contextually, as it can act as a decay-promoting factor or a stabilizer depending on cellular signals and binding partners. Dysregulation of KHSRP is implicated in neurodegenerative diseases, muscle atrophy, and viral infections, where its RNA-regulatory functions are hijacked or disrupted.
KHSRP antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blot, immunoprecipitation, and immunofluorescence to investigate KHSRP's involvement in RNA metabolism pathways. Validated antibodies help dissect its dual roles in mRNA decay and miRNA maturation, offering insights into disease mechanisms and therapeutic targets. Commercial KHSRP antibodies are typically raised against specific epitopes, with validation in knockout models to ensure specificity.
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