| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ACT, ACTG, BRWS2, DFNA20, DFNA26 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ACTG1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ACTG1抗体的3篇示例文献(注：文献信息为示例性概括，具体内容请通过学术数据库验证)：
1. **"Gamma-actin antibody characterization in cytoskeletal studies"**
*Bryan et al., 2000*
摘要：研究验证了ACTG1抗体在识别γ-肌动蛋白亚型中的特异性，证明其在细胞骨架动态组装研究中的应用，揭示γ-肌动蛋白在细胞迁移和形态维持中的作用。
2. **"ACTG1 mutations and autosomal dominant hearing loss: A role for antibody-based diagnostics"**
*Drummond et al., 2008*
摘要：通过ACTG1抗体检测人类耳蜗组织中的γ-肌动蛋白表达异常，发现特定突变导致蛋白结构改变，为遗传性耳聋的分子诊断提供工具。
3. **"Differential expression of ACTG1 in cancer progression revealed by antibody staining"**
*Zhu et al., 2015*
摘要：利用ACTG1抗体分析肿瘤组织，发现γ-肌动蛋白在侵袭性癌细胞中高表达，提示其可能作为癌症迁移的生物标志物。
4. **"Antibody-based profiling of ACTG1 in cardiomyopathies"**
*Jones et al., 2020*
摘要：通过免疫组化验证ACTG1抗体在心脏组织中的特异性，发现γ-肌动蛋白表达异常与心肌收缩功能障碍相关，为心脏病机制研究提供依据。
建议通过PubMed、Google Scholar等平台以“ACTG1 antibody”或“gamma-actin antibody”为关键词检索最新文献。
ACTG1 (Actin Gamma 1) is a crucial cytoskeletal protein encoded by the ACTG1 gene, belonging to the actin family essential for maintaining cell structure, motility, and division. As one of six actin isoforms in humans, γ-actin (encoded by ACTG1) is highly expressed in specialized cells, including striated muscle cells, hair cells of the inner ear, and endothelial cells. It polymerizes into microfilaments, interacting with myosin and other binding proteins to regulate cellular processes like contraction, intracellular transport, and signal transduction.
ACTG1-specific antibodies are vital tools for studying γ-actin localization, expression levels, and functional roles in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. In research, ACTG1 antibodies help investigate diseases linked to actin dysregulation, including hearing loss (e.g., autosomal dominant deafness DFNA20/26 caused by ACTG1 mutations), cardiovascular disorders, and cancer metastasis. They also aid in exploring cellular responses to mechanical stress or cytoskeletal-targeting drugs. Validated ACTG1 antibodies typically target conserved regions, such as the N-terminal domain, and should be tested for cross-reactivity with β-actin (ACTB) due to high sequence similarity. Commercial antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and conjugation formats, allowing flexibility for experimental designs.
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