| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/5000 | Human,Mouse,Rat |
| Aliases | MGF, STAT5 |
| WB Predicted band size | 91 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human STAT5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于STAT5A抗体的3篇文献示例(内容为模拟概括，仅供参考)：
1. **"Generation and characterization of a monoclonal antibody specific for STAT5A"**
*Wakao, H., et al. (1995).*
摘要：该研究开发了一种针对STAT5A蛋白的单克隆抗体，验证了其在Western blot和免疫沉淀中的特异性，并用于检测STAT5A在细胞信号传导中的激活状态。
2. **"STAT5A phosphorylation status correlates with prognosis in breast cancer"**
*Lin, J.X., et al. (2000).*
摘要：通过STAT5A特异性抗体分析乳腺癌样本，发现STAT5A的持续磷酸化与患者预后不良相关，提示其在肿瘤进展中的潜在作用。
3. **"STAT5A regulates T-cell development via antibody-mediated pathway analysis"**
*Moriggl, R., et al. (2005).*
摘要：利用STAT5A抗体研究其在T细胞分化中的功能，发现STAT5A缺失导致胸腺细胞发育异常，强调其在免疫系统中的关键性。
4. **"STAT5A knockout mice reveal its role in mammary gland development"**
*Hennighausen, L., et al. (1997).*
摘要：通过抗体检测STAT5A在乳腺组织中的表达，结合基因敲除模型，证明STAT5A对泌乳相关基因的调控至关重要。
(注：以上信息为示例性概括，实际文献需通过PubMed/Google Scholar等平台检索确认。)
**Background of STAT5A Antibody**
The STAT5A antibody is a crucial tool in molecular and cellular biology research, specifically targeting the Signal Transducer and Activator of Transcription 5A (STAT5A) protein. STAT5A, a member of the STAT family, plays a pivotal role in the Janus kinase (JAK)-STAT signaling pathway, which regulates gene expression in response to cytokines, growth factors, and hormones. It is involved in critical cellular processes, including proliferation, differentiation, apoptosis, and immune responses.
STAT5A is activated via tyrosine phosphorylation, typically by JAK kinases, leading to its dimerization, nuclear translocation, and binding to specific DNA sequences to modulate target gene transcription. Dysregulation of STAT5A is linked to cancers (e.g., leukemia, breast cancer), immune disorders, and hematopoietic defects.
The STAT5A antibody is widely used to detect STAT5A expression, phosphorylation status, and localization in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. It helps distinguish STAT5A from its closely related isoform, STAT5B, which shares ~96% sequence identity but exhibits distinct tissue-specific functions and regulatory roles.
Researchers employ STAT5A antibodies to study its role in oncogenesis, immune cell signaling, and developmental biology, as well as to evaluate therapeutic targets in diseases with aberrant STAT5 activation. Specificity validation (e.g., knockout controls) is essential to ensure accurate detection, given the high homology within the STAT family.
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