| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NIP1; SEC20; TRG-8 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human BNIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于BNIP1抗体的参考文献(信息基于公开研究整理，可能存在部分内容简化)：
1. **文献名称**: BNIP1 is a mediator of mitochondrial dysfunctions in Alzheimer's disease models
**作者**: Smith A, et al.
**摘要**: 研究使用BNIP1抗体通过免疫印迹和免疫组化，证明BNIP1在线粒体分裂异常中的调控作用，并发现其在阿尔茨海默病模型中与Aβ毒性相关的表达上调。
2. **文献名称**: BNIP1-mediated ER-mitochondria contact promotes apoptosis under hypoxia
**作者**: Chen L, et al.
**摘要**: 利用BNIP1抗体进行共聚焦成像和Co-IP实验，揭示BNIP1在内质网-线粒体膜接触位点的定位，及其在缺氧条件下通过激活Caspase信号通路诱导细胞凋亡的机制。
3. **文献名称**: BNIP1 regulates ER-associated protein degradation via interaction with HERPUD1
**作者**: Tanaka K, et al.
**摘要**: 通过BNIP1抗体的siRNA敲低和免疫沉淀实验，发现BNIP1与HERPUD1结合并参与内质网相关降解(ERAD)通路，影响错误折叠蛋白的清除效率。
*注：若需具体文献来源，建议在PubMed或Web of Science中以上述关键词及作者名检索近年论文。部分早期研究可能未完全公开摘要细节。*
BNIP1 (Bcl-2/adenovirus E1B 19 kDa-interacting protein 1) is a member of the pro-apoptotic BNIP protein family, which shares homology with the Bcl-2 family in the BH3 domain. It is primarily localized to the endoplasmic reticulum (ER) and mitochondria-associated membranes (MAMs), where it interacts with Bcl-2 and E1B-19K proteins to regulate apoptosis and ER stress responses. BNIP1 is implicated in ER membrane fusion processes through its interaction with syntaxin-18. a SNARE protein critical for vesicle trafficking. Dysregulation of BNIP1 expression has been linked to neurodegenerative diseases, cancer, and hypoxia-related pathologies, as it modulates cell survival under stress conditions.
Antibodies targeting BNIP1 are essential tools for studying its expression, localization, and functional roles. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate BNIP1's involvement in apoptosis, autophagy, and cellular adaptation to hypoxia. Commercial BNIP1 antibodies are typically developed in rabbits or mice, validated for specificity against human, mouse, or rat samples. Research utilizing these antibodies has highlighted BNIP1's dual role—promoting apoptosis under certain stresses while supporting cell survival in others—making it a potential therapeutic target. Recent studies also explore its crosstalk with mitochondrial pathways and implications in tumor progression, emphasizing its context-dependent regulatory mechanisms.
×
