| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AIF; AUNX1; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6; SEMDHL |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human AIFM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AIFM1抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*AIF deficiency induces oxidative stress and cell death in human cells*
**作者**：Vahsen, N. et al.
**摘要**：本研究利用AIFM1抗体通过Western blot和免疫荧光技术，揭示AIFM1缺失导致线粒体功能障碍和活性氧积累，最终引发caspase非依赖性细胞死亡，为神经退行性疾病机制提供依据。
2. **文献名称**：*Interaction between AIF and CHCHD4 regulates respiratory chain biogenesis*
**作者**：Hangen, E. et al.
**摘要**：通过共免疫沉淀实验(使用AIFM1抗体)，作者发现AIFM1与线粒体膜蛋白CHCHD4的相互作用对呼吸链复合物组装至关重要，突变导致能量代谢异常，提示其在疾病中的潜在作用。
3. **文献名称**：*Mutations in the mitochondrial apoptotic pathway factor AIFM1 cause X-linked recessive Charcot-Marie-Tooth disease*
**作者**：Rinaldi, C. et al.
**摘要**：该研究在遗传性周围神经病变患者中发现AIFM1突变，通过免疫组化结合AIFM1抗体证实突变导致蛋白表达异常及轴突退化，建立了基因型与表型的直接关联。
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**备注**：上述文献为示例性质，实际引用时建议通过PubMed或Google Scholar核对最新研究。AIFM1抗体常用于检测蛋白表达、定位及相互作用，是研究线粒体凋亡通路的重要工具。
The AIFM1 (Apoptosis-Inducing Factor Mitochondrial 1) antibody targets a protein encoded by the *AIFM1* gene, which plays a critical role in mitochondrial energy metabolism and programmed cell death. AIFM1 is a flavoprotein located in the mitochondrial intermembrane space, functioning primarily as an NADH oxidase essential for oxidative phosphorylation. Beyond its metabolic role, AIFM1 is a key mediator of caspase-independent apoptosis. Upon cellular stress, it translocates to the nucleus, triggering chromatin condensation and DNA fragmentation. Mutations in *AIFM1* are linked to severe human disorders, including X-linked Charcot-Marie-Tooth disease, mitochondrial encephalopathy, and neurodevelopmental defects, highlighting its importance in neuronal and muscular health.
AIFM1 antibodies are widely used in research to study protein expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help investigate AIFM1’s dual roles in cell survival and death, particularly in neurodegenerative diseases, cancer, and ischemia-reperfusion injury. Commercially available antibodies are typically raised in rabbits or mice, targeting specific epitopes across AIFM1’s functional domains. Validation includes knockout controls to ensure specificity. Recent studies also explore AIFM1’s involvement in redox signaling and inflammatory responses, expanding its therapeutic relevance. Thus, AIFM1 antibodies serve as vital tools for unraveling mitochondrial biology and disease mechanisms.
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