| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | K18; CYK18 |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human KRT18 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于KRT18抗体的3篇示例参考文献(内容为虚构，仅作格式示例)：
---
1. **文献名称**: *KRT18 as a Biomarker for Circulating Tumor Cells in Colorectal Cancer*
**作者**: Lambrechts, D. et al.
**摘要**: 本研究利用KRT18抗体检测外周血中的循环肿瘤细胞(CTCs)，发现其表达水平与结直肠癌患者的转移风险显著相关。通过免疫荧光和流式细胞术验证，证明KRT18抗体可作为预后评估工具。
---
2. **文献名称**: *Phosphorylated KRT18 in Hepatocellular Carcinoma Diagnosis*
**作者**: Schutte, B. et al.
**摘要**: 研究通过KRT18抗体分析肝细胞癌(HCC)组织样本，发现磷酸化KRT18的异常表达与肿瘤分化程度相关。免疫组化结果显示，KRT18抗体能区分不同HCC亚型，提示其在病理分型中的应用潜力。
---
3. **文献名称**: *KRT18 Cleavage as an Apoptosis Marker in Chemotherapy*
**作者**: Leers, M. et al.
**摘要**: 本文开发了一种基于KRT18抗体的ELISA方法，用于检测caspase裂解的KRT18片段(CK18-Asp396)。研究证明该标记物可动态监测化疗诱导的肿瘤细胞凋亡，与患者治疗反应呈正相关。
---
*注：以上文献为示例，非真实存在。实际引用时需检索PubMed等数据库获取真实研究。*
Keratin 18 (KRT18) is a type I intermediate filament protein encoded by the *KRT18* gene, primarily expressed in single-layered epithelial cells (e.g., liver, intestine, glandular epithelia). It pairs with type II keratin KRT8 to form intermediate filaments, providing structural support and maintaining cellular integrity. KRT18 is involved in stress response, organelle organization, and apoptosis. Its phosphorylation during cellular stress (e.g., drug toxicity, viral infection) modulates these processes.
In pathology, KRT18 is a biomarker for epithelial-derived cancers (e.g., carcinomas) and liver diseases. Cleaved KRT18 fragments released during apoptosis are detectable in serum, aiding in monitoring disease progression (e.g., via M30/M65 ELISA assays). Antibodies against KRT18 are widely used in research (Western blot, immunohistochemistry, immunofluorescence) to study epithelial differentiation, cancer metastasis, and drug responses. They also aid in diagnosing tumors of uncertain origin and assessing liver injury (e.g., viral hepatitis, fibrosis). Mutations in *KRT18* are linked to cryptogenic cirrhosis and other disorders, highlighting its clinical relevance. Overall, KRT18 antibodies are essential tools for exploring epithelial biology and disease mechanisms.
×
