| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human VIM |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与VIM(波形蛋白)抗体相关的经典研究文献示例，涵盖基础生物学及疾病机制方向：
---
1. **文献名称**: *Vimentin: The Conundrum of the Intermediate Filament Gene Family*
**作者**: Franke, W.W., et al.
**摘要**: 该综述系统总结了波形蛋白(Vimentin)作为中间丝蛋白家族成员的结构特征、细胞功能及其在胚胎发育、细胞迁移中的作用，并讨论了其抗体在细胞分型研究中的应用价值。
---
2. **文献名称**: *Vimentin in Cancer and Its Potential as a Molecular Target for Therapy*
**作者**: Satelli, A., & Li, S.
**摘要**: 研究揭示了肿瘤细胞中Vimentin表达与上皮-间质转化(EMT)及转移的关系，利用特异性抗体证实其作为转移性癌症的生物标志物，并探讨靶向Vimentin的治疗策略。
---
3. **文献名称**: *Role of Vimentin in Immune Cell Recruitment and Lung Fibrosis*
**作者**: Mendez, M.G., et al.
**摘要**: 通过基因敲除和抗体阻断实验，发现Vimentin调控纤维化模型中免疫细胞浸润，提示其抗体可能用于抑制促纤维化信号通路，为抗纤维化药物开发提供依据。
---
如需特定领域(如神经科学或自身免疫疾病)的文献，可进一步说明以调整检索方向。实际文献请通过PubMed或Google Scholar核实。
Vimentin antibodies are immunological tools targeting vimentin, a type III intermediate filament protein expressed in mesenchymal cells. Vimentin plays crucial roles in maintaining cell integrity, organelle positioning, and cellular migration. It is dynamically involved in processes like mitosis, cell signaling, and stress responses. As a marker of epithelial-to-mesenchymal transition (EMT), vimentin overexpression is associated with cancer metastasis, fibrosis, and chronic inflammation, making it a research focus in oncology and immunopathology.
Vimentin antibodies are widely used in research to study cytoskeletal reorganization during EMT, tumor progression, and wound healing. In diagnostics, they help identify mesenchymal-derived cells in tissue samples, aiding cancer subtyping and stromal analysis. Notably, autoantibodies against vimentin have clinical significance in autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus, where they contribute to pathogenesis through molecular mimicry or post-translational modifications.
Commercial vimentin antibodies are validated for techniques including immunofluorescence, Western blotting, and flow cytometry. Challenges remain in specificity due to vimentin's structural homology with other intermediate filaments. Recent studies explore its extracellular roles in bacterial adhesion and viral entry, expanding potential therapeutic applications. Proper antibody validation remains critical, as non-specific binding can lead to misinterpretation in both research and clinical settings.
×
