Rabbit Polyclonal BID Antibody

BID (BH3-interacting domain death agonist) is a pro-apoptotic protein belonging to the BCL-2 family, which regulates mitochondrial pathways of programmed cell death. It exists as an inactive cytosolic monomer until proteolytically cleaved by caspase-8 during apoptosis signaling, generating truncated BID (tBID). This activated form translocates to mitochondria, where it interacts with BAX/BAK to induce outer membrane permeabilization, triggering cytochrome c release and caspase cascade activation. BID antibodies are essential tools for detecting BID expression, activation, and localization in apoptosis studies. They are widely used in techniques like Western blot, immunohistochemistry, and immunofluorescence to investigate BID's role in physiological apoptosis and pathological conditions, including cancer, neurodegenerative diseases, and ischemia-reperfusion injury. Specific antibodies targeting distinct epitopes (full-length BID, tBID, or phosphorylated forms) enable researchers to dissect BID's dual roles in death receptor-mediated apoptosis and DNA damage responses. Recent studies also explore BID's non-apoptotic functions in cell cycle regulation and inflammation, expanding the utility of BID antibodies in diverse research contexts.