| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP28 |
| Uniprot No | Q9H239 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 123-520aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSFAKQGNKWYKQHLSYRLVNWPEHLPEP AVRGAVRAAFQLWSNVSALEFWEAPATGPADIRLTFFQGDHNDGLGNAFD GPGGALAHAFLPRRGEAHFDQDERWSLSRRRGRNLFVVLAHEIGHTLGLT HSPAPRALMAPYYKRLGRDALLSWDDVLAVQSLYGKPLGGSVAVQLPGKL FTDFETWDSYSPQGRRPETQGPKYCHSSFDAITVDRQQQLYIFKGSHFWE VAADGNVSEPRPLQERWVGLPPNIEAAAVSLNDGDFYFFKGGRCWRFRGP KPVWGLPQLCRAGGLPRHPDAALFFPPLRRLILFKGARYYVLARGGLQVE PYYPRSLQDWGGIPEEVSGALPRPDGSIIFFRDDRYWRLDQAKLQATTSG RWATELPWMG CWHANSGSALF |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP28重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*MMP-28. a novel human matrix metalloproteinase with a unique domain structure and potential substrate specificity*
**作者**：Llano, E., Pendás, A.M., Freije, J.P., et al.
**摘要**：该研究首次克隆并表达了人源MMP28重组蛋白，发现其具有独特的结构域(含类Ig结构域)，并通过酶活性实验表明其可能特异性切割细胞外基质成分(如明胶和酪蛋白)，提示其在组织重塑中的潜在作用。
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2. **文献名称**：*Epilysin (MMP-28) promotes macrophage apoptosis through cleavage of α5 integrin*
**作者**：Manicone, A.M., Harju-Baker, S., & McGuire, J.K.
**摘要**：研究利用重组MMP28蛋白处理巨噬细胞，发现其通过切割α5整合素触发细胞凋亡，揭示了MMP28在炎症消退中的调控机制，为靶向免疫治疗提供新思路。
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3. **文献名称**：*MMP-28 modulates epithelial-mesenchymal transition in airway epithelial cells*
**作者**：Gharib, S.A., Johnston, L.K., & Huizar, I.
**摘要**：通过体外实验表达重组MMP28蛋白，证明其可诱导气道上皮细胞发生上皮-间质转化(EMT)，提示MMP28在哮喘和肺纤维化等疾病中可能促进组织修复或病理纤维化。
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**备注**：若需更多文献，可进一步检索近年研究，如MMP28在肿瘤微环境或病毒感染中的重组蛋白功能分析(例如*Journal of Biological Chemistry*或*Matrix Biology*期刊相关论文)。
**Background of MMP28 Recomcombinant Protein**
Matrix metalloproteinase 28 (MMP28), also known as epilysin, is a member of the MMP family, zinc-dependent endopeptidases involved in remodeling extracellular matrix (ECM) components and regulating cell signaling. Unlike many MMPs, MMP28 is expressed constitutively in healthy tissues, particularly during embryonic development, and persists in specific adult tissues like the skin, lungs, and testes. It is synthesized as an inactive zymogen with a propeptide domain, a catalytic domain, and a hemopexin-like domain, requiring proteolytic cleavage for activation.
MMP28 plays roles in tissue homeostasis, wound repair, and immune modulation. Studies suggest it processes ECM proteins (e.g., fibronectin) and cytokines (e.g., TGF-β), influencing epithelial-mesenchymal interactions and inflammation. Its dysregulation is linked to fibrotic diseases, cancer progression, and chronic inflammatory conditions, though its precise mechanisms remain less defined compared to other MMPs.
Recombinant MMP28 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its structure, substrate specificity, and biological functions. The recombinant form often includes tags (e.g., His-tag) for purification via affinity chromatography. Researchers utilize it to investigate enzymatic activity, inhibitor screening, and signaling pathways in vitro or in animal models.
Current research focuses on clarifying MMP28's dual roles in tissue repair versus pathological remodeling, aiming to explore its therapeutic potential. As a recombinant tool, it aids in deciphering MMP28's contributions to developmental biology and disease, offering insights for targeting MMP28 in fibrosis or cancer therapies. However, challenges remain in understanding its context-dependent functions and optimizing recombinant protein stability for functional assays.
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