| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP14 |
| Uniprot No | P50281 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 112-288aa |
| 氨基酸序列 | YAIQGLKWQHN EITFCIQNYT PKVGEYATYE AIRKAFRVWE SATPLRFREV PYAYIREGHE KQADIMIFFA EGFHGDSTPF DGEGGFLAHA YFPGPNIGGD THFDSAEPWT VRNEDLNGND IFLVAVHELG HALGLEHSSD PSAIMAPFYQ WMDTENFVLP DDDRRGIQQL YGGESG |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP14重组蛋白的3篇参考文献示例(文献信息为模拟生成，仅供参考)：
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1. **文献名称**: *"Recombinant MMP14 expression and functional characterization in collagen remodeling"*
**作者**: Smith J, et al.
**摘要**: 研究报道了人源MMP14重组蛋白在大肠杆菌中的表达与纯化，并验证其体外水解胶原蛋白的活性，揭示其在细胞外基质重塑中的关键作用。
2. **文献名称**: *"MMP14 recombinant protein promotes tumor invasion via activation of pro-MMP2 in breast cancer models"*
**作者**: Lee H, et al.
**摘要**: 通过哺乳动物细胞系统表达MMP14重组蛋白，证明其通过激活MMP2前体蛋白促进乳腺癌细胞侵袭，为靶向MMP14的癌症治疗提供依据。
3. **文献名称**: *"Structural insights into MMP14 catalytic domain by recombinant protein crystallization"*
**作者**: Zhang R, et al.
**摘要**: 利用昆虫表达系统获得高纯度MMP14催化结构域重组蛋白，通过X射线晶体学解析其三维结构，揭示底物结合位点特征，助力抑制剂开发。
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注：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实文献。如需具体文献推荐，建议补充研究背景或应用方向。
**Background of MMP14 Recombinant Protein**
Matrix metalloproteinase 14 (MMP14), also known as membrane-type 1 MMP (MT1-MMP), is a member of the zinc-dependent endopeptidase family. It plays a pivotal role in extracellular matrix (ECM) remodeling by degrading components like collagen, gelatin, and fibronectin. Unlike many secreted MMPs, MMP14 is a transmembrane protease anchored to the cell surface, enabling localized ECM degradation and regulation of cellular interactions with the microenvironment.
MMP14 is crucial for physiological processes, including tissue development, wound healing, and angiogenesis. However, its dysregulation is implicated in pathological conditions such as cancer metastasis, fibrosis, and inflammatory diseases. Notably, MMP14 activates pro-MMP2 (a key gelatinase) through proteolytic cleavage, amplifying ECM degradation cascades. This makes it a focal point in studying tumor invasion and metastasis.
Recombinant MMP14 protein is produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to enable controlled studies of its structure, function, and inhibition. The recombinant form typically includes the catalytic domain to retain enzymatic activity while excluding transmembrane regions for solubility. Purification tags (e.g., His-tag) facilitate isolation, and activity is often validated using fluorogenic substrates or gelatin zymography.
Research applications include screening MMP14 inhibitors for therapeutic development, modeling ECM remodeling in 3D cell cultures, and investigating its role in disease mechanisms. Recombinant MMP14 also serves as a critical tool for structural studies (e.g., crystallography) to design targeted therapies. Despite challenges in maintaining native conformation and activity *in vitro*, advances in protein engineering continue to enhance its utility in both basic research and drug discovery.
In summary, MMP14 recombinant protein bridges understanding of ECM biology and disease, offering insights for therapeutic strategies targeting proteolytic pathways.
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