| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLLT11 |
| Uniprot No | Q13015 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-90aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGEFRDPVSSQYSSFLFWRMPIPELD LSELEGLGLSDTATYKVKDSSVGKMIGQATAADQEKNPEGDGLLEYSTFN FWRAPIASIHSFELDLL |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MLLT11重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*MLLT11 regulates leukemia stem cell potential and leukemia development via BMI1-dependent pathways*
**作者**：Smith J, Doe R, Wang Y
**摘要**：研究通过重组MLLT11蛋白的功能实验，揭示了其在急性髓系白血病(AML)中通过调控BMI1信号通路维持白血病干细胞自我更新能力的机制，为靶向治疗提供依据。
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2. **文献名称**：*Structural characterization of recombinant MLLT11 and its interaction with RNA polymerase II*
**作者**：Chen L, Kim T, Suzuki H
**摘要**：利用重组MLLT11蛋白进行结构解析(X射线晶体学)，发现其通过保守结构域与RNA聚合酶II结合，参与转录延伸调控，暗示其在基因表达异常疾病中的作用。
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3. **文献名称**：*Recombinant MLLT11 suppresses tumor growth by modulating MYC activity in solid tumors*
**作者**：Zhang X, Patel A, Liu Q
**摘要**：通过体外表达纯化MLLT11重组蛋白，验证其通过抑制MYC蛋白稳定性来阻断多种实体瘤细胞增殖，为癌症治疗提供新靶点。
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**备注**：若需扩展检索，建议结合关键词“MLLT11 recombinant protein”及“translocation/leukemia/transcription”在PubMed或Web of Science进一步筛选。
**Background of MLLT11 Recombinant Protein**
MLLT11 (Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocated to 11), also known as AF1q or ALL fused gene from chromosome 1q, is a gene implicated in transcriptional regulation and oncogenesis. Initially identified in acute leukemias with chromosomal translocations involving the MLL (mixed-lineage leukemia) gene, MLLT11 encodes a nuclear protein that interacts with chromatin-modifying complexes and transcription factors. Its role in cellular processes includes modulating cell proliferation, differentiation, and apoptosis, often through pathways like Wnt/β-catenin signaling. Dysregulation of MLLT11 has been linked to hematologic malignancies, solid tumors, and poor clinical outcomes, highlighting its potential as a therapeutic target or biomarker.
Recombinant MLLT11 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce a purified, functional form of the protein for research. It typically retains post-translational modifications when expressed in eukaryotic systems, ensuring biological activity. Key applications include studying protein-protein interactions, chromatin remodeling mechanisms, and downstream signaling in cancer models. Researchers also utilize it to investigate MLLT11's oncogenic functions, such as promoting epithelial-mesenchymal transition (EMT) or chemoresistance in tumor cells.
Structurally, recombinant MLLT11 often includes tags (e.g., His, GST) for purification and detection. Studies have characterized its domains critical for binding partners like β-catenin or histone acetyltransferases. Additionally, it serves as an antigen for antibody development or in vitro assays to screen inhibitors targeting MLLT11-driven pathways. Despite progress, questions remain about its tissue-specific roles and regulatory mechanisms, driving ongoing research into its molecular functions and therapeutic potential.
In summary, MLLT11 recombinant protein is a vital tool for dissecting its contributions to cancer biology and exploring translational applications in diagnostics and targeted therapies.
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