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Human Insulin-like growth factor-binding protein 7 (IGFBP7/MAC25/PSF) ELISA kit

  • 中文名： 人胰岛素样生长因子结合蛋白7(IGFBP7/MAC25/PSF) ELISA kit
货号: IPD-E17249h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人胰岛素样生长因子结合蛋白7(IGFBP7/MAC25/PSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、匀浆样本中的IGFBP7含量。IGFBP7 即胰岛素样生长因子结合蛋白 7，它在细胞生长、增殖等过程中发挥关键作用。作为新兴靶点，其研究机制主要聚焦于与肿瘤发生发展、转移及预后的关联，还涉及对血管生成的调控等，有望为相关疾病治疗提供新思路。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。广泛应用于肿瘤生物学研究、器官纤维化机制探索（如肝纤维化、肺纤维化）以及代谢性疾病相关的基础研究，为揭示IGFBP7在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AGM ELISA Kit; Angiomodulin ELISA Kit; FSTL2 ELISA Kit; IBP-7 ELISA Kit; IBP7 ELISA Kit; IBP7_HUMAN ELISA Kit; IGF binding protein 7 ELISA Kit; IGF-binding protein 7 ELISA Kit; IGFBP rP1 ELISA Kit; IGFBP-7 ELISA Kit; IGFBP-rP1 ELISA Kit; IGFBP7 ELISA Kit; IGFBPRP1 ELISA Kit; Insulin like growth factor binding protein 7 ELISA Kit; Insulin-like growth factor-binding protein 7 ELISA Kit; MAC25 ELISA Kit; MAC25 protein ELISA Kit; PGI2 stimulating factor ELISA Kit; PGI2-stimulating factor ELISA Kit; Prostacyclin stimulating factor ELISA Kit; Prostacyclin-stimulating factor ELISA Kit; PSF ELISA Kit; RAMSVPS ELISA Kit; TAF ELISA Kit; Tumor-derived adhesion factor ELISA Kit
  • 缩写：
    IGFBP7
  • Uniprot No.：
    Q16270
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, homogenates
  • 检测范围：
    78 pg/ml - 5000 pg/ml
  • 灵敏度：
    19.5 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)639.284625.027610.845619.564629.406635.987SD0.0400.0360.0350.0500.0560.049CV(%)5.0834.6514.5996.5027.1986.250
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP7/MAC25/PSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:10

    Average %

    97


    Range %

    87-105


    1:20

    Average %

    97


    Range %

    90-105


    1:40

    Average %

    99


    Range %

    91-108


    1:80

    Average %

    96


    Range %

    90-102









  • 回收率：

    The recovery of human IGFBP7/MAC25/PSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    90-98


    EDTA plasma (n=4)

    97

    90-103









  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.405

    2.412

    2.409

    2.231


    2500

    1.895

    1.876

    1.886

    1.708


    1250

    1.246

    1.324

    1.285

    1.107


    625

    0.753

    0.769

    0.761

    0.583


    312

    0.444

    0.457

    0.451

    0.273


    156

    0.345

    0.360

    0.353

    0.175


    78

    0.210

    0.200

    0.205

    0.027


    0

    0.172

    0.184

    0.178











  • 货期：
    3-5 working days

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