| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; HLHS1; PPKCA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GJA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于GJA1(Connexin43)抗体的3篇参考文献示例(注：以下为模拟内容，建议通过学术数据库检索真实文献)：
---
1. **标题**: *"Altered Connexin43 Expression in Ischemic Cardiomyopathy: Implications for Gap Junction Remodeling"*
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组织化学和Western blot技术，利用GJA1特异性抗体分析了心肌梗死患者心脏组织中Cx43的分布和表达水平，发现缺血区域Cx43表达显著下调且空间排列紊乱，提示其与心律失常的发生相关。
2. **标题**: *"GJA1 Antibody-Based Detection of Connexin43 in Cutaneous Wound Healing"*
**作者**: Lee B, et al.
**摘要**: 通过GJA1抗体标记小鼠皮肤伤口模型，发现Cx43在表皮角质形成细胞中的动态变化，其表达降低可加速伤口闭合，表明靶向Cx43可能成为促进组织修复的策略。
3. **标题**: *"Connexin43 Modulates Glioblastoma Invasion via Antibody-Validated Protein Interactions"*
**作者**: Zhang X, et al.
**摘要**: 研究采用GJA1抗体进行免疫沉淀和共聚焦成像，揭示了Cx43在胶质母细胞瘤细胞侵袭中的双重作用，其表达水平与肿瘤细胞迁移能力呈负相关。
---
**建议**：可通过PubMed或Google Scholar搜索关键词“GJA1 antibody”、“Connexin43 antibody + application”(如“cancer”或“cardiac”)获取真实文献。部分经典研究可能涉及抗体验证(如宿主物种、克隆号)及在疾病模型中的应用。
The GJA1 antibody targets the Gap Junction Alpha-1 protein, commonly known as Connexin 43 (Cx43), a member of the connexin family that forms gap junction channels. These channels facilitate direct intercellular communication by allowing the exchange of ions, metabolites, and signaling molecules between adjacent cells, critical for coordinating cellular activities in tissues. GJA1/Cx43 is widely expressed in various organs, including the heart, brain, skin, and bone, playing roles in cardiac conduction, neural signaling, wound healing, and skeletal development.
Antibodies against GJA1 are essential tools in biomedical research for detecting Cx43 expression, localization, and post-translational modifications (e.g., phosphorylation) that regulate channel gating and turnover. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study gap junction dynamics in health and disease. Dysregulation of Cx43 is linked to pathologies such as arrhythmias, neurodegenerative disorders, cancer metastasis, and skin diseases. For instance, reduced Cx43 expression in cardiac tissue is associated with ischemic injury, while aberrant expression in tumors may influence cell proliferation and invasion.
Researchers also utilize GJA1 antibodies to explore therapeutic strategies targeting gap junctions, such as enhancing communication in damaged tissues or inhibiting it in cancer. Specificity validation (e.g., knockout controls) is crucial due to potential cross-reactivity with other connexins. Overall, these antibodies provide insights into cell-cell communication mechanisms and their translational applications.
×
